CALCIFIED TISSUE INTERNATIONAL: INSTRUCTIONS FOR AUTHORS

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1 CALCIFIED TISSUE INTERNATIONAL: INSTRUCTIONS FOR AUTHORS TYPES OF MANUSCRIPT SUBMISSIONS COPYRIGHT INFORMATION ONLINE SUBMISSION VIA SCHOLARONE MANUSCRIPT PREPARATION GUIDELINES FOR ELECTRONICALLY PRODUCED FIGURES AFTER ACCEPTANCE TYPES OF MANUSCRIPT SUBMISSIONS ORIGINAL ARTICLES should report studies originated by the author and should contain important new information of relevance to the aims and scope of the journal. Word limit 5000 words, 45 references, no more than 8 figures/tables. LETTERS TO THE EDITOR should be short comments to published articles. Word limit 500 words. REVIEWS on controversial issues are accepted only upon invitation by one Editor. Word limit words, 100 references, no more than 10 figures. COPYRIGHT INFORMATION Submission of a manuscript implies: that the work described has not been published before (except in the form of an abstract or as part of a published lecture, review, or thesis); that it is not under consideration for publication elsewhere; that its publication has been approved by all coauthors, if any, as well as by the responsible authorities at the institute where the work has been carried out; that, if and when the manuscript is accepted for publication, the authors agree to automatic transfer of the copyright to the publisher; that the manuscript will not be published elsewhere in any language without the consent of the copyright holders; that written permission of the copyright holder is obtained by the authors for material used from other copyrighted sources; and that any costs associated with obtaining this permission are the authors' responsibility. ONLINE SUBMISSION VIA SCHOLARONE Manuscripts are submitted online to Calcified Tissue International via ScholarOne. Please log-in directly onto the site at for online manuscript submission. Manuscripts sent via regular or electronic mail will not be considered for review and will be returned to the authors. If you are unable to submit your manuscript via ScholarOne, please contact the editorial office: Roberto Civitelli, M.D. Washington University School of Medicine Division of Bone and Mineral Diseases 660 South Euclid Avenue Campus Box 8301 St Louis, Missouri 63110, USA Phone: Fax: cti@dom.wustl.edu

2 Authors entering the journal's ScholarOne site must have a user account. If you have an existing account, for example if you have submitted to Calcified Tissue International before via ScholarOne, use your existing account for all your submissions and to track their status in real time online. If you are unsure about whether or not you have an account, or have forgotten your password, enter your address into the "Password Help" section to the right of the homepage. If you do not have an account, click on the "Create Account" link in the home page. Once you have logged into your account, ScholarOne will lead you through the submission process in a step-by-step orderly process, from the Dashboard of the Author Center. If you cannot finish your submission in one visit, you can save a draft and re-enter the process at the same point for that manuscript. Authors will be required to enter the following information into ScholarOne: Full title of the manuscript The first and last names of all contributing authors and academic degrees [i.e., first name, middle initial(s), surname, degree(s)]; the departmental and institutional affiliation(s); complete street or mailing address for each affiliation, including the city, state or province, and country The complete name and address of the author to whom correspondence should be sent, as well as his/her phone number, fax number, and address. Please, note that the corresponding (or submitting) author is not automatically listed as a co-author of the manuscript. If, as in most circumstances, the corresponding author is also a co-author, please ensure that the name is entered first in the author list. A short title for use as a running head. The abstract of the manuscript. This should be identical to the abstract included in the main manuscript file. However, this copy of the abstract is only for review purposes. If there are discrepancies between what has been entered in ScholarOne during the submission process and what is contained in the electronic files submitted, the latter will prevail. Only the submitted electronic files (manuscript and figure files) will be used for publication. Before starting the submission process, ensure that you have all your electronic material ready. Please, carefully read the section on MANUSCRIPT PREPARATION, below to ensure that all the required information and documents are prepared in the format accepted by the journal. At any point during the submission process there are Help buttons available to see common questions and a support link to ask a specific question via . Keep copies of your word-processing and graphics files. If you are submitting electronic graphics that you have scanned, be prepared to send the hard copy originals upon request. While the electronic files you have created are satisfactory for the review process, they may not be of sufficient quality for printing. This also holds true for files created in low-resolution graphics environments such as PowerPoint, etc. After submission, you may return periodically and monitor the progress of your submission through the review process.

3 MANUSCRIPT PREPARATION Manuscripts must be clearly and concisely written in English. They should be carefully scrutinized for errors before they are submitted. Correctness of spelling, grammar, and typing is the responsibility of the author. Foreign contributors, whose first language is not English, should enlist the help of colleagues who are proficient in scientific English. Laboratory slang, clinical jargon, and colloquialisms are not acceptable. The Editors reserve the right to edit accepted manuscripts. TEXT FORMATTING: The main document with manuscript text and tables should be prepared with an electronic word processing program. Please, do NOT include figures or illustrations in the manuscript text file. If you use Microsoft Word, the system can directly convert the Word formatted file, including all special characters. If you use other word processing software, such as WordPerfect, files should be saved in RTF format. RTF (Rich Text Format) is a common export property of most popular word processors. Check your word processor to see if it can export or "Save As" your file in RTF format. MS Word and WordPerfect both contain this function.. Use a normal, plain font (e.g., 11-point Times Roman) for text. Use the automatic page numbering function to number the pages. Do not use field functions. Use tab stops or other commands for indents, not the space bar. Use the table function, not spreadsheets, to make tables. Use the equation editor or MathType for equations. Note: If you use Word 2007, do not create the equation with the default equation editor but use the Microsoft equation editor or MathType instead. Save your file in.doc format. Do not submit.docx files. You can also use the Springer Word template [ID , ] Manuscripts with mathematical content can also be submitted in LaTeX. LaTeX macro package [ID , 0] For review purposes, your text and figure file(s) will be converted into HTML so that they can be easily viewed with an internet browser. They will also be converted into a PDF document so that they can be viewed and printed with Adobe Acrobat Reader. The files in the.pdf document will be presented in the order specified. TITLE PAGE: The title page should include: the name(s) of the author(s), a concise and informative title, the affiliation(s) of the author(s) and the address, address, telephone, and fax numbers of the corresponding author. ABSTRACT: This should not exceed 250 words and is of great importance to readers. It should describe briefly in complete sentences the purpose of the investigations, the methods utilized, the results

4 obtained, and the authors' principal conclusions. The abstract must be easily understood without recourse to the text or list of references. Avoid non-standard abbreviations, unfamiliar terms, symbols or acronyms not easily understood by the general scientific reader. The abstract must follow the title page. You will be requested to enter a copy of the abstract in ScholarOne during the online submission process. This copy of the abstract is for review purposes only. KEY WORDS: Up to 5 key words characteristic of the contents and relevant field must be entered. INTRODUCTION: The introduction orients the reader in respect to the state of knowledge in the specific area under investigation. It assumes that the reader has knowledge of mineral and calcified tissue metabolism. It should cite recent important work by others. Repetition of information in the introduction and the discussion is undesirable. EXPERIMENTAL SUBJECTS: For clinical studies, patient and control populations should be described in detail. In many studies, details of age, race, and sex are important. In experiments involving human subjects, it should be stated that informed consent was obtained from the subjects and that the investigations had been approved by an institutional human research committee. Safeguards for protection of the rights of minors and mentally defective subjects should be stated. Reviewers are asked to verify whether the manuscript contains satisfactory information indicating human studies approval by the appropriate institutional authorities. Editors will decline manuscripts in which such approval is not clear or missing. In text, tables, and figures, patients should be identified by number or serial letter rather than by initials or names. Photographs of patients' faces should be included only if scientifically relevant and the identity of the patient should be concealed by masking. Authors must obtain written consent from patients and/or from their Institution s human research committee for use of such photographs. There is increasing concern about the accurate and timely reporting of results from clinical trials, especially those that are sponsored by pharmaceutical companies or device manufacturers. To ensure the integrity of the process of publishing clinical trial results, authors wishing to publish such results in Calcified Tissue International should adhere to the principles and standards published by the American Association of Medical Colleges, available at: Calcified Tissue International fully endorses such principles and standards. MATERIALS AND METHODS: These should be described and referenced in sufficient detail so that other workers can repeat your study. The sources of unusual chemicals and reagents and special pieces of apparatus should be stated. For modified methods, only the modification used needs to be described. Experiments involving animals must be approved by the appropriate institutional animal care and oversight committee. The authors are responsible for providing assurance that their experimental procedures involving animals are in compliance of the Guiding Principles in the Care and Use of Animals, approved by the Council of the American Physiological Society in As for the human studies, reviewers and specifically asked to verify adherence to such principles. Editors will decline manuscripts in which compliance is not clear or missing.

5 RESULTS: The experimental data should be presented briefly in text, tables, or figures, but the authors should avoid redundant methods of presentation as much as possible. DISCUSSION: Avoid repetition of material in the introduction and detailed repetition of the experimental findings. The discussion should be focused on the experimental findings and their interpretation. Unsubstantiated speculations and plans for future study are not appropriate. FIGURES: The importance of clear figures cannot be over-emphasized and great care should be exercised in their preparation. Legends should be brief, self-sufficient explanations of the figures in no more than four or five lines. Please, see GUIDELINES FOR ELECTRONICALLY PRODUCED FIGURES, below, for details on how to produce high quality electronic illustrations. If you are asked to produce paper copies of your illustrations upon acceptance, please consider that these must exactly reproduce the illustrations and figures in the accepted version of the manuscript. No changes are allowed to a manuscript that has been accepted. In preparing publication quality prints, please ensure that halftone figures are sharp, well-contrasted prints, trimmed at right angles. The publisher reserves the right to reduce or enlarge illustrations. With X-rays, please indicate significant parts on the back of the copy, or on an overlay. Inscriptions on halftones should be approximately 3 mm high. For line drawings, please submit good-quality glossy or laser prints. The inscriptions should be clearly legible with words in upper-and lowercase characters, not all capitals. Recommended sizes are: 2 mm for capital letters and numbers, and 1.6 mm for lowercase letters. Cost of Color Figures: Color can be used without charge for the electronic edition of the journal but will appear in the printed version of the journal at the author's expense. The cost for color reproduction in the printed journal is 950/$1150 per figure, charged to the author. TABLES: Use one page for each table and number them using Arabic numerals. Each table must have a concise heading (title) explaining its content, and must be intelligible without reference to the text. As with figures, tables should always be cited in text in consecutive numerical order. If necessary, identify any previously published material by giving the original source in the form of a reference at the bottom of the table. ACKNOWLEDGEMENTS: Acknowledgements of people, grants, funds, etc. should be placed in a separate section before the reference list. The names of funding organizations should be written in full. DISCLOSURE OF FINANCIAL CONFLICTS OF INTEREST: In a section immediately following Acknowledgements, authors must indicate whether or not they have a financial relationship with the organization that sponsored the research, or with any other organizations whose area of activity may be affected or perceived to be influenced by the results of the study. A financial relationship may be in the form of honoraria received for consultancy or speaking activities on behalf of such organizations, or for participation to advisory boards; stock ownership; grants received for research projects; royalties; or part-time of full-time employment in that organization. The authors should also state that they have full control of all primary data and that they agree to allow the journal to review their data if requested. When reporting results of clinical trials, the specific role of each author in the work reported should be explained in detail. If the manuscript is accepted, a slightly edited version of the disclosure statement will be published as a footnote in the first page of the article.

6 In addition to the disclosure statement, each author must sign and submit an Authorship/Disclosure Form. The form may be uploaded during the submission process through ScholarOne, or faxed to the North American Editorial office at , or sent by in pdf format to REFERENCES: The author is responsible for the accuracy of the references. These must be cited in numerical order [in brackets] in the text with only one reference to a number. References to articles in journals must be given in the following order: names of all authors with initials, year, title of paper with only initial letter of initial word capitalized, abbreviated journal name using Index Medicus form followed by volume number and first and last page of article. Journal title abbreviations will be those used in Index Medicus, listed in the first section of the "Subject Index" in each cumulated volume. Journal titles will be cited as they existed at the time of publication. For books: Author's last name with initial(s), year of publication, title of book, publisher, and city of publication. Examples: (1) Journal Article: Name(s) and initials of all author(s), year in parentheses, full title, journal name as abbreviated in Index Medicus, volume followed by a colon, first and last pages numbers. Peppler WW, Mazess RB (1981) Total body bone mineral and lean body mass by dual-photon absorptiometry. I. Theory and measurement procedure. Calcif Tissue Int 33: (2) Articles from Electronic Publications: Name(s) and initials of all author(s), year in parentheses, full title, journal name as abbreviated in Index Medicus, DOl number, and publication date. lijima M, Moradian-Oldak J (2004) Interactions of Amelogenins with Octocalcium Phosphate Crystal Faces Are Dose Dependent. Calcif Tissue Int, DOl: /s , January 23, 2004 (3) Book: Name(s) and initials of all author(s), year in parentheses, title, edition, publisher, place of publication. Chayen J, Bitensky L, Butcher RG (1973) Practical histochemistry. John Wiley & Sons, London, New York (4) Chapters in Books: Name(s) and initials of all author(s), year in parentheses, title of chapter. In: names and initials of all editors, title of book, publisher, place of publication, first and last page numbers. Byers P (1977) The diagnostic value of bone biopsies. In: Avioli LV, Krane SM (eds) Metabolic bone disease. Academic Press, New York London, p An article should be listed in the references as "in press" only when the journal which has accepted it can be named; if it has not yet reached this stage, it should not appear in the reference list but the author(s') name(s) in the text should be followed by: (in preparation). "Personal Communications" should be treated in the same way. FIGURE LEGENDS: Include figure legends in the text file of the manuscript, not in the figure file (see GUIDELINES FOR ELECTRONICALLY PRODUCED FIGURES for further details).

7 ABBREVIATIONS AND SYMBOLS: Abbreviations should be defined at first mention and used consistently thereafter. Abbreviations and symbols acceptable by the journal must conform to the recommendations of the International Union of Pure and Applied Chemistry (IUPAC) and the International Union of Biochemistry and Molecular Biology (IUBMB). A list of abbreviations, symbols, and nomenclature is published in: Biochemical Nomenclature and Related Documents, 2nd edition, Portland Press Editor C. Liebecq. Drugs must be designated by their generic names. Trade names spelled exactly as trademarked and with the initial letter capitalized may be used after the drug has been identified once by its generic name or by its systematic chemical name. Terminology for bone histomorphometry should conform to the nomenclature, symbols, and units detailed in the Report of the American Society of Bone and Mineral Research Committee, published in: J. Bone Miner. Res. 2: ; GUIDELINES FOR ELECTRONICALLY PRODUCED FIGURES For production of the figures of an accepted paper, the Springer production department requires electronically produced figures to be stored in either EPS (Encapsulated PostScript) or TIFF (Tag Image File Format) format. Most drawing programs (e.g., Adobe Illustrator, Deneba Canvas, Corel Draw) have a Save-As -EPS option in their Save dialog box. Other information about EPS can be found at the Adobe website. You can verify the suitability of your electronic images for printing by using Digital Expert, an electronic tool available on the Sheridan Group web site, at If your images are not adequate for printing, you will have to provide paper printed artwork. GENERAL Upload figures separately from the text (i.e. files should not be integrated with the text files). Unless the author(s) have specified they wish to pay for color illustrations, color figures will be converted to grey scale in the print edition. Most presentation programs (Excel, PowerPoint, Freelance) produce data that cannot be stored in an EPS or Tiff format. Therefore graphics produced by these programs cannot be used for print. VECTOR (LINE) GRAPHICS Vector graphics exported from a drawing program should be stored in EPS format only. Suitable drawing program: Adobe Illustrator. For simple line art, the following drawing programs are also acceptable: Corel Draw, Freehand, Canvas. No rules narrower than.25 point. No grey screens paler than 15% or darker than 60%. Screens meant to be differentiated from one another must differ by at least 15%. SPREADSHEET/PRESENTATION GRAPHICS Most presentation programs (Excel, PowerPoint, Freelance) produce data that cannot be stored in an EPS or TIFF format. Therefore graphics produced by these programs cannot be used for print. HALFTONE FIGURES Black & white and color figures should be saved in EPS or TIFF format.

8 Figures should be created using Adobe Photoshop whenever possible. SCANS Scanned reproductions of black and white photographs should be provided as 300 ppi EPS or TIFF files. Scanned color figures should be provided as EPS or TIFF files scanned at a minimum of 300 ppi with a 24-bit color depth. Line art should be provided as EPS or TIFF files at 600 ppi. FIGURE LETTERING To add lettering, it is best to use Helvetica or Arial (sans serif) fonts. Keep lettering consistently sized throughout your final-sized artwork, usually about 2-3 mm (8-12pt). Variance of type size within an illustration should be minimal, e.g., do not use 8-pt type on an axis and 20-pt type for the axis label. Avoid effects such as shading, outline letters, etc. Do not include titles or captions in the illustration. FIGURE NUMBERING All figures are to be numbered using Arabic numerals. Figures should always be cited in the text in consecutive numerical order. Figure parts should be denoted by lowercase letters (a, b, c, etc.) Name your figure files with Fig and the figure number and type of file, e.g., Fig1.eps. FIGURE LEGENDS Each figure should have a concise caption describing accurately what the figure depicts. Include the captions in the text file of the manuscript, not in the figure file. Figure captions begin with the term Fig. in bold type, followed by the figure number, also in bold type. No punctuation is to be included after the number, nor is any punctuation to be placed at the end of the caption. Identify all elements found in the figure in the figure caption; and use boxes, circles, etc., as coordinate points in graphs. Identify previously published material by giving the original source in the form of a reference citation at the end of the figure caption. PERMISSIONS If figures included have already been published elsewhere, permission must be obtained from the copyright owner(s) for both the print and online format. Please be aware that some publishers do not grant electronic rights for free and that Springer will not be to refund any costs that may have occurred to receive these permissions. In such cases, material from other sources should be used. ELECTRONIC SUPPLEMENTARY MATERIAL: Electronic supplementary material will be published in the online version only. It may consist of Information that is more convenient in electronic form: sequences, spectral data, etc. Large original data, e.g. additional table, illustrations, etc.

9 To accommodate user downloads, please keep in mind that larger-sized files may require very long download times and that some users may experience other problems during downloading. Electronic supplementary material will be published as received from the author without any conversion, editing, or reformatting. NUMBERING If supplying and supplementary material, the text must make specific mention of the material as a citation, similar to that of figures and tables. Refer to the supplementary files as Online Resource, e.g.,...additional data are given in Online Resource 2. Name the files consecutively. LEGENDS For each supplementary material, please supply a concise caption describing the content of the file. AFTER ACCEPTANCE: Upon acceptance of your article you will receive a link to the special Author Query Application at Springer s web page where you can sign the Copyright Transfer Statement online and indicate whether you wish to order Open Choice, offprints, or printing of figures in color. Once the Author Query Application has been completed, your article will be processed and you will receive the proofs. OPEN CHOICE In addition to the normal publication process (whereby an article is submitted to the journal and access to that article is granted to customers who have purchased a subscription), Springer provides an alternative publishing option: Springer Open Choice. A Springer Open Choice article receives all the benefits of a regular subscription-based article, but in addition is made available publicly through Springer s online platform SpringerLink. We regret that Springer Open Choice cannot be ordered for published articles. Springer Open Choice [ COPYRIGHT TRANSFER Authors will be asked to transfer copyright of the article to the Publisher (or grant the Publisher exclusive publication and dissemination rights). This will ensure the widest possible protection and dissemination of information under copyright laws. Open Choice articles do not require transfer of copyright as the copyright remains with the author. In opting for open access, they agree to the Springer Open Choice Licence. NIH COMPLIANCE NIH grantees or recipients of grants from other funding bodies which require your article to be archived in an Open Access repository such as PubMed Central can view Springer s policy here. OFFPRINTS

10 Additional offprints can be ordered by the corresponding author. COLOR ILLUSTRATIONS Online publication of color illustrations is free of charge. For color in the print version, authors will be expected to make a contribution towards the extra costs. PROOF READING The purpose of the proof is to check for typesetting or conversion errors and the completeness and accuracy of the text, tables and figures. Substantial changes in content, e.g., new results, corrected values, title and authorship, are not allowed without the approval of the Editor. After online publication, further changes can only be made in the form of an Erratum, which will be hyperlinked to the article. ONLINE FIRST The article will be published online after receipt of the corrected proofs. This is the official first publication citable with the DOI. After release of the printed version, the paper can also be cited by issue and page numbers

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