Author Guidelines for Preparing Manuscripts

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Author Guidelines for Preparing Manuscripts For the Proceedings of the Association for Biology Laboratory Education (ABLE) These guidelines were prepared to assist presenters of major workshops, mini-workshops, and posters in the preparation of their manuscripts for publication in the ABLE workshop/conference proceedings, Tested Studies for Laboratory Teaching. Although it is not possible to provide guidelines for every usage, this document attempts to address the most common questions. All manuscripts are to be prepared by copying and pasting your manuscript text to the ABLE Proceedings Template. This template is already formatted to follow our guidelines. Submission of Manuscript Your manuscript is due to the Proceedings Editor no later than the October 1st immediately following the annual conference. (Of course, your submission is very welcome any time before that date!) All manuscripts must be submitted electronically using the ABLE Proceedings Template via the ABLE manuscript submission website. See the pull-down menu under Proceedings, Author Guidelines, and click on Manuscript Submission. Please submit the following: One electronic copy of your complete manuscript (following the guidelines below), sent as an uploaded file (Microsoft Word file of the ABLE Proceedings Template; PC or Mac versions) on the Manuscript Submission webpage found on the ABLE website. Signed ABLE Copyright Form from the submitting author. The submitting author represents all the authors of the manuscript and must submit a copyright form listing all the authors and the copyright owner. Copyright forms are available for download from the ABLE web site. The Copyright Form is a fill-able pdf document. Type the names of all the authors, type the name of the copyright owner(s), list the year as the year following the conference presentation, print a copy and sign on behalf of all the authors

2 ABLE Manuscript Guidelines scan and create a new pdf, and upload the scanned Copyright Form pdf at the ABLE Proceedings Manuscript Submission Webpage when you submit your manuscript. Please enter all the information requested in the on-line Manuscript Submission webpage. Some of this information will be redundant with the content of your manuscript (see Format of Proceedings Manuscripts below) but is needed by our website managers to efficiently catalog your manuscript once the Proceedings are published on our website. If a manuscript is not submitted for a conference presentation, the conference program abstract for that contribution will be published. Format of Proceedings Manuscripts The format described below applies to all major workshop manuscripts. Presenters of miniworkshops may submit either an extended abstract (up to two pages with tables and/or figures) or a short ( 10 page) manuscript following the same format as major workshop manuscripts (described below). In the absence of either an extended abstract or a short manuscript submission, the mini-workshop abstract that was printed in the conference program will be published. Presenters of posters may submit their poster file (pdf or ppt) and either an extended abstract (up to two pages with tables and/or figures) or a short ( 10 page) manuscript following the same format as major workshop manuscripts (described below). In the absence of a poster submission, the poster abstract that was printed in the conference program will be published in an abstracts only section at the end of the Proceedings. Please submit your manuscript adhering to the following format (use the current ABLE Proceedings Template available from the ABLE website which is pre-set to the format below). To preserve the template formatting, use Paste and Match Format in the Edit menu (Mac) or Paste Merge Formats in the Edit menu (Windows). The template has comments to help guide your through the process of correctly formatting your manuscript. You may delete the template comments as your correctly format each component of your manuscript. The format specification also are described below: The beginning of your article includes the following: title in 20-point bold Times New Roman font, align left authors in 14-point bold Tines New Roman, align left mailing address in 12-point Times New Roman for each author, align left. List institution, department, street address, city state or province (as abbreviation), mailing code, country (e.g., USA, CA) email address in 12-point bold Times New Roman for each author, align left an abstract (in 10-point Times New Roman), written as a single paragraph (not to exceed 150 words), align left keywords (3-8) in 10-point Times New Roman copyright line: year of publication (year after conference in which workshop was presented), copyright holder s name (author/s or institution) in 10-point Times New Roman, align left contribution type: short title entered in the header starting on page 2 that will appear on even-numbered pages in 8-point Times New Roman, align right. The contribution types are Major Workshop, Mini-Workshop or Poster. authors last names: entered in the header starting on page 3 that will appear on oddnumbered pages in 8-point Times New Roman, align left. Enter the author last names in the same order as on the title page.

Primary Headings: Enter a line break after each heading. ABLE Manuscript Guidelines 3 Introduction: A general introduction, written for the instructor, should provide the objectives of the laboratory exercise and contain relevant background information (e.g., level of difficulty, time required to prepare and set up, time required for students to perform) to assist the reader in assessing how the exercise can best be used. This introduction is distinct from that in the Student Outline. Student Outline: This section should contain the text that is distributed to the students. Materials: A list of materials and equipment required by students in the laboratory (specify quantities as per number of students). In short manuscripts for mini-workshops and poster presentations, the Materials section may be very brief and abbreviated. Notes for the Instructor: This section should highlight critical steps in the experimental procedure and issues relating to safety in the laboratory. Remember that many of the persons who will attempt to use your exercise may have little or no expertise in the topic(s) covered. Lacking your knowledge and experience, they may encounter problems which are not apparent in your description of the Student Outline. Address any problems the technicians had in preparing for your workshop and the major questions and problems participants had during your workshop. Detailed instructions should be included in appendices. Acknowledgments: Acknowledge those who contributed to the exercise. Cited References: Include only those references cited in the text. Use the name-year citation format in the text of the manuscript and list these cited references in Cited References alphabetically. We are using the Council of Science Editors (CSE) style for in-text citations and Cited References formats starting in 2016. References given as further reading should also be cited in the body of the text. For example: For further reading see Allen (1981), Baker and Carpenter (1976), Dodds et al. (1985), and Street (1973, 1989). so they may be included in the list of Cited References. About the Author(s): A short biography should be written in paragraph form and typically includes academic training, current position, extraordinary awards or achievements, and research and teaching interests. Appendices: Include addresses of suppliers, instructions for the preparation of media and chemicals, rearing of animals, growing of plants, and any other special instructions and information. If there is more than one appendix refer to them as APPENDIX A, APPENDIX B, etc., and give each a concise title. Supplemental materials such as website contents or prepared text materials may be submitted separate from your Proceedings manuscript via the ABLE Manuscript Submission website. After editorial review, supplemental materials may be posted to the ABLE Proceedings website and linked to your Proceedings chapter. Generally, short manuscripts for mini-workshops and posters do not include Appendices.

4 ABLE Manuscript Guidelines Manuscript Preparation Use the ABLE Proceedings Template for the correct format Base font: 10-point Times New Roman Line space: Single, enter a line break after each primary, secondary and tertiary heading. Margins: 1.25 top; 1.00 bottom; 0.75 both sides Justification: Full Columns: The Introduction is two column format, Student Outline is one column format and the remainder of the manuscript, starting with Materials is two column format. Sections: The Student Outline begins on a new page and ends on a page separate from Materials. Pagination: Page numbers are pre-set in the Footer, right justified (in the ABLE Proceedings Tempate) and every manuscript will have independent pagination starting with page 1. Authors do not enter page numbers in the Footer. Headings (as described in the ABLE Proceedings Template) In general use no more than four levels of organization: primary headings (centered and bold in 12-point Times New Roman font), secondary headings (left-hand margin and bold 11- point), tertiary headings (left-hand margin and in italic 11-point), and quaternary (left-hand margin, underlined, and in 11 point). It is not necessary to have secondary headings unless complexity of the text calls for them; tertiary headings may be used directly under primary headings if they introduce limited material. Body or paragraph Indent the first line of each paragraph 1/2 inch (0.50 ). Use full justification and do not break words at the right-hand margin anywhere in the manuscript. (This includes hyphenated words as well as words divided between syllables.) Give the scientific and colloquial names the first time a plant or animal is mentioned. Scientific names should appear in italics and not be underlined. Spelling should follow American English (e.g., behavior, center, color, liter, pipet). For general matters of spelling use Webster's New Collegiate Dictionary; if two or more spellings of a word are given, the first listed is the one preferred (with the exception of formulae and appendices). Use 10-point Times New Roman as the base font. All portions of the manuscript should be single-spaced, including text, titles of tables, figure legends, references cited, and appendices. However, insert a single line break before and after each primary, secondary and tertiary heading. Consult the ABLE Proceedings Template to guide you in this formatting. Tables When possible, use the TABLE feature in MS Word to create your tables. Please do NOT use spaces or tabs to align the columns of your table. Identify each table with a bold numeric reference and title, centered at the top of the table (see example below). When possible, the table should be centered within the page. Table 6. Number of aquatic insects in stream sample. Pollution-intolerant Intermediate Pollution-tolerant caddisfly larvae dobsonfly larvae mayfly larvae beetle larvae crane flies damselflies blackfly larvae midge larvae

ABLE Manuscript Guidelines 5 Graphics Embed each graphic in your document as a graphic file. Include a centered figure legend below the graphic (using narrower margins on both sides), as shown in the example below. Figure 1. Typical results for simulated growth of catfish as a function of protein content of the diet. Growth was simulated in tanks with temperature set at 25 C, oxygen set at 10 mg/liter, and with a feeding rate which was varied to match the feed consumption rate of the fish. Documentation and Cited References When citing references in the text, use the form Jones (1981) if the author's name is part of the sentence and (Jones, 1981) if it is not. If there are more than two authors use the form: Barnes et al. (1984) ; however, use all names in the Cited References. Provide multiple citations in alphabetical order; for example, Ashe (1967), Ball (1945), and Cook (1987). Provide page number(s) for material that is quoted directly from a source. For example: Haldane (1927:21) once wrote comparative anatomy is largely the story of the struggle to increase surface in proportion to volume. We are using the Council of Science Editors (CSE) style for text citations and Cited References formats starting in 2016. Additional references and further readings should be cited directly within the body of the text or appear in an appendix. Pay particular note of the use of punctuation and capital letters in the samples below; periods that follow name(s), year, title and journal name are followed by two blank spaces. In all book citations include the total number of pages. Some examples of Cited References entries are given below: Books: Lane N. 2009. Life ascending: the ten great inventions of evolution. New York (NY): Norton. 344 p. Sokal RR, Roth FJ. 1973. Introduction to biostatistics. San Francisco: Freeman. 368 p. On-line Journal Articles, including ABLE Proceedings, include website address and/or DOI: Beck CW, Migabo S, Blumer LS. 2011. Substrate size selection by bean beetles. In: McMahon K, editor. Tested studies for laboratory teaching. Volume 32. Proceedings of the 32 nd Workshop/Conference of the Association for Biology Laboratory Education (ABLE). p. 25-31. http://www.ableweb.org/volumes/vol-32/v32reprint.php?ch=3 Journal Articles (use ISI journal title abbreviations): Bednarski AE, Elgin SCR, Pakrasi HB. 2003. An inquiry into protein structure and genetic disease: introducing undergraduates to bioinformatics in a large introductory course. CBE Life Sci Educ. 4:207-220. Breslin PAS, Beauchamp GK. 1997. Salt enhances flavour by suppressing bitterness. Nature. 387:563. Hanauer DI, Jacobs-Sera D, Pedulla ML, Cresawn SG, Hendrix RW, Hatfull GF. 2006. Teaching scientific inquiry. Science. 314:1880-1881.

6 ABLE Manuscript Guidelines Copyright The manuscript must be accompanied by letters of permission from copyright holders to include any copyrighted material in your manuscript. Style and Usage Do not use underlining anywhere in the text; scientific names should be in italics. Hyphenate compounds used as adjectives (3-year-old male, 10-mL pipet, 3-minute intervals). Use serial commas, including that preceding the conjunction (left, right, and center; not: left, right and center). Leave one space after punctuation that ends sentences (periods, question marks, etc.) and only one space after colons, semicolons, commas, and other internal punctuation marks. Numbers and Mathematics Use decimals rather than fractions, except in equations. Decimals not preceded by a whole number should always be preceded by a zero (0.75). Use numerals for numbers greater than nine except when starting a sentence (associated abbreviation or symbol should be spelled out as well: Eleven grams, but About 11 g ; Fifteen percent, but More than 15% ). Spell out numbers one through nine except when used with units of measure or time (6 mm, 3 days, 4 years, but five students, seven observations), or in a series that includes at least one number greater than nine (1 syringe, 3 beakers, and 25 test tubes). Use commas in numbers of four digits or more (1,000) except in catalog numbers. In giving ranges, use from 10.1 to 31.0 mm, but the range is 10.1 31.0 mm (i.e., use to when it is preceded by from ). Specify currency as U.S. or Canadian dollars ($40 US, $48 CDN). Units of Measure Use the metric system for all measures (20 kg, 6 g, 30 km). A list of accepted abbreviations that can be used in text, tables, and figures of manuscript, without explanation or punctuation, appears below. Other measures should be spelled out (e.g., dozen, day, inch, second, minute, hour, month, week, year). C degrees Celsius m meter mm millimeter cm centimeter µg microgram M molar g gram µl microliter X magnification kg kilogram ml milliliter nm nanometer km kilometer mg milligram Miscellaneous Italicize all Latin words and abbreviations, except those commonly used (e.g., i.e.). Periods and commas should be placed within closing quotation marks. A semicolon and colon should be placed outside closing quotation marks. Final Check Before Submission Authors are strongly advised to carefully review their manuscript to check that it follows the ABLE Proceedings format and to ensure that the manuscript has the authors intended placement of Tables and Figures. To aid in your final review, save your manuscript as a pdf document and then check the placement of Tables and Figures. Placements may change if there is text that provides website addresses. Text that describes html hyperlinks will be converted to

ABLE Manuscript Guidelines html link text (blue active link text) and text following it will shift up. This problem may be corrected by placing extra line breaks prior to the text (Figure legend) that is shifting, so text following an image cannot shift above a Figure. The submitting author will receive a manuscript proof (pdf document) from the ABLE Proceedings Editor for review and approval prior to on-line publication. 7 Last Revised: 24 August 2016