Introduction to Thermal Stimulator in ABL

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Author, date: Veli-Matti Saarinen, 6.7.2018 Introduction to Introduction to in ABL 1 Introduction These instructions are to provide information to about how to work with the Somedic MSA Thermal Stimulator in ABL. More information about the systems can be found from system manuals, which can be asked from the personnel of ABL. (Thermode 381) and SenseLab software are installed in the DC-room. Synchronization with Stimulus PC is implemented over serial cable. MSA is based on Peltier element ; when applying a voltage on a thermocouple it causes a temperature difference between the junctions of those two metals. 00076 AALTO Espoo abl-sci@aalto.fi 1/8

2 Wiring and components Equipment of Thermal stimulator Product Image Location Stim PC Control Room MSA Control Room Thermode (30x30mm) SenseLab Modular Sensory Analyzer Article nr: 10-010-0001 S/N: 381 DC-Room / Control room SenseLab optic modem Thermode 381 Control room - DC-shelf MSA Optic receiver MSA Fiber Optic/Serial 1/f Article number: 10-010-7010 S/N: 180 2/8 00076 AALTO Espoo abl-sci@aalto.fi

Button DC-Room / Control room Toolkit Control room - AC-shelf Image 1: Thermal stimulator wiring in ABL The MSA is located outside the shielded room. The wire of the Thermode goes through the feedthrough panel into the DC-Room. Cable of the response button is connected to BNC connector on MSA front panel and goes through feedthrough panel filter. 00076 AALTO Espoo abl-sci@aalto.fi 3/8

Image 2: Front panel of MSA. Pat 1 = BNC connector of the response button. T and R = optic connectors for Optic receiver. Thermode and two white connectors at the bottom are for Thermode connectors. Image 3: Optic fiber is connected to Optic Receiver. 00076 AALTO Espoo abl-sci@aalto.fi 4/8

Optic fiber has two optic fibers, one with black connectors and one with red connectors. The cable should be connected so that T -connector on MSA is connected to Rx of Optic Receiver and Tx -connector of Optic Receiver is connected on R connector of MSA. The labels refer to Transmitter and Receiver. Optic receiver is connected to Stimulus PC via serial cable (USB-serial cable) to COM3 port. 2.1) Details Somedic MSA thermal stimulator (Somedic SenseLab AB, Norra Mellby, Sweden) with a 25 x 25 mm Peltier element Thermode (baseline 32 C, max slope 5 C/s, range 5-52 C). 3 Before measurements 3.1) Fluid levels MSA Thermode is based on Peltier element. The system uses fluid circuit, which evaporates over time from the pipelines. doesn t work properly with low fluid; therefore it needs to be monitored and refilled occasionally. Amount of liquid can be checked inside the device after opening the cover by loosening two screws on the back (top). Image 4: Cover can be opened by loosening upper corner screws from the back. The white cap of the bottle can be seen on the left middle. Check the liquid level from the marker tape on the side of the bottle. There are six lines on the tape. If the liquid goes under line 3 (from the bottom), the bottle should be refilled. Bottle is full when the liquid reaches the top line. More liquid can be found from the refrigerator of the 4 th floor kitchen. 00076 AALTO Espoo abl-sci@aalto.fi 5/8

Liquid can be added using the syringe found from the toolkit. Image 5: Check the liquid level from the tape markers on the side of the bottle. Add log information about the refill to MSA Log book. Please contact personnel, if you need any help. 4 Stimulus PC MSA can be controlled from Stimulus PC, using Somedic s own SenseLab software, or by controlling through SDK of the MSA. For example Presentation software has been used previously in ABL. MSA is controlled over serial port, more precisely COM3 of STIM PC. 4.1) SenseLab 00076 AALTO Espoo abl-sci@aalto.fi 6/8

SenseLab software is installed on Stimulus PC and can be run by clicking Sense650 icon. SenseLab communicates with MSA through serial port. USB-serial port converter is connected to USB port on Stimulus PC and other end is connected to optic receiver. Open Com Port window by choosing Control-> Com port window and choose COM 3. Define experiment sequence from Sequence-> Settings. Sequences can be set from Sequence window. Subject needs to be also defined before measurement. 4.2) Presentation Set the following serial port settings when using presentation. If you are interested, please ask more details from personnel. 00076 AALTO Espoo abl-sci@aalto.fi 7/8

, 5 Measuring Place the wanted skin area on top of the Thermode. Subjects can be instructed to push the response button to test first change in temperature, first sensation of pain or pain tolerance. Be careful not to induce too much heat for too long time for same skin area. Even a red skin is already a burn. Baseline of the Thermode is 32 C, range 5-52 C, slope between 1-5 C/s. 00076 AALTO Espoo abl-sci@aalto.fi 8/8