PINMRF Checkout Quiz - Varian Inova-300 Version Please carefully read every question and select the best answer(s). Some questions may have more than one correct answer. You must select all the correct answers to receive credit. You must answer at least 28 questions correctly to pass (ca. 80 %). This is an open-book quiz. You may use any training materials and notes that you have. You have 30 minutes to complete this exam. Name: Group: Date: Trained by: e-mail: Signature: By signing above you certify that you will abide by the PINMRF rules and regulations, and that you have understood the safety-related material that has been presented to you. 09-06-2011: Update - JSH 11-29-2007: Update - JSH 08-30-2006: First release JSH
1. Which of the following lock functions need to be optimized before locking your sample? A. Field and lock gain. B. Sweep rate, lock gain and lock power. C. Shims, lock gain and lock phase. D. Field, lock gain, and lock phase. E. None of them relevant lock functions are set by using the correct h1xxxx command. 2. Which of the following lock functions may need to be optimized after locking your sample? A. Spin rate and lock gain. B. Lock gain and lock power. C. Lock gain and lock phase. D. Field and lock gain. E. None of them you should never adjust any lock function, period. 3. When should you adjust the lock phase? A. Never. B. When the lock level is too low. C. When the lock level is too high. D. When the shimming is poor. E. When you are bored. 4. NMR samples are spun for what purpose? A. To obtain a uniform mixture of solute and the solvent. B. To obtain a lock signal. C. To bring the signal on resonance with the transmitter frequency. D. To obtain a more uniform average magnetic field over the sample volume and therefore lower resolution and sensitivity. E. To obtain a more uniform average magnetic field over the sample volume and therefore higher resolution and sensitivity. 5. If your sample will not spin properly, what should you do? A. Increase the spin rate setting. B. Eject and re-insert the sample several times to clean out the system. C. Run your experiment without spinning if the locking and shimming are OK. D. Remove your sample and transfer the solution to another NMR tube. E. Turn up the air pressure using the regulator close to the spectrometer. F. Check the spinning using the lock standard sample if it is still not good, notify PINMRF staff. 1
6. If your sample tube breaks with all or part of the tube in the NMR magnet, what should you do? A. Pick up any glass outside the magnet, hide the spinner, and leave the lab as quickly as possible. B. Remove the probe from the magnet, and then clean out the glass from the probe. C. Put a note on the spectrometer that a sample is broken in the magnet. D. Notify PINMRF staff that a sample has broken, giving details of the sample composition. E. Turn up the air pressure, using the regulator close to the spectrometer, to blow out any glass. 7. On the PINMRF Varian Inova-300 spectrometers, what do the following commands mean? nt wft ga print rt rts standard aph zg exit 8. The term FID stands for A. Fourier transformed Induced Decay B. Free Induction Decay C. Free Incoherent Decoupling D. Fourier Inductance Decay E Ferrous Incoherent Device 9. For PINMRF's 300 & 400 MHz spectrometers, how many 15-minute blocks of NMR time between the hours of 9:00 a.m to 5:00 p.m. may an individual reserve using the written sign-up sheets? A. One per day B. Two per day C. Four per day C. As many as you like, any day 2
10. Which of the following statements are true regarding the saving and storage of NMR data? A. You are allowed to save NMR data on the PINMRF Varian spectrometers. B. You are NOT allowed to save NMR data on the PINMRF Varian spectrometers. C. PINMRF staff will archive your saved NMR for you. D. PINMRF staff will NOT archive your saved NMR for you. 11. When saving NMR data files, which of the following regarding naming files is correct? A. you can use any characters in the filename, up to 80 characters. B. you can use letters and numbers and the space bar in the filename. C. you can use letters and numbers and the dash and the underscore in the filename. D. you must not use capital letters as part of the filename. 12. The signal-to-noise ratio of a spectrum is proportional to the square root of the number of scans acquired. Therefore, if you have run a 13 C experiment for four hours, and would like to increase the existing signal-to-noise ratio by a factor of two, how much longer will you need to acquire data for? A. four hours. B. eight hours. C. twelve hours. D. sixteen hours. 13. If the volume of your sample is ca. 500ul (not the standard 700 800 ul) which of the following figures below represents the correct sample position in the sample depth gauge? A. B. C. D. 3
14. What benefit(s) are obtained by using the sample depth gauge to center the sample volume in the probe coil? A. It puts the sample in the most homogeneous magnetic field, which improves lineshape. B. The sample will spin more easily due to its mass centered around the coil. C. Sensitivity is improved because the most sample is within the probe coil. D. The z shims have been optimized for this position of the sample, so shimming is easier. E. B and D only. F. A and C only. G. A, C and D. H. A, B, C and D. 15. When running more than one sample on a Varian spectrometer, you must A. tune, match, and shim every sample. B. spin, calibrate the pulse width, and lock every sample. C. lock and shim every sample. D. use the depth gauge, lock, and shim every sample. E. be very patient. 16. Safety regulations prohibit persons with pacemakers or magnetic objects inside the line of a superconducting magnet. A. 50 Gauss B. 10 Gauss C. 5 Gauss D. 2 Gauss E. 1 Gauss 17. Any user-specific parameters can be changed A before the standard parameters have been read. B. after the standard parameters have been read. C. never parameters should not be changed. D. none of the above. 18. Which of the following statements are correct regarding probe tuning on the PINRMF Varian Inova-300 NMR Spectrometers? A. The user must always tune the probe for every sample. B. The user does not need to tune the probe when running 1 H, 19 F, 31 P or 13 C experiments. C. The user does need to tune the probe for nuclei other than 1 H, 19 F, 31 P or 13 C. D. Probe tuning on the Varian requires additional training. E. 11 B spectra can be obtained on the Inova-300-2 spectrometer (4100 BRWN) without having to tune the probe. F. All choices except A are correct. 4
19. In order to autophase your spectrum, type A. apk. B. aph. C. phase. D. you must always manually phase your spectrum. 20. What is the proper method to insert the sample tube into the spinner? A. Hold the large end of the spinner in your hand and push the sample tube into the small end of the spinner. B. Hold the small end of the spinner in your hand and push the sample tube into the large end of the spinner. C. Put the spinner in the depth gauge and push the sample tube into the large end of the spinner. D. Hold the large end of the spinner in your hand and push the sample tube into the large end of the spinner. 21. The PINMRF Varian Inova-300 s do have sample-temperature controllers. This statement is A. TRUE. B. FALSE. 22. NEVER place an empty spinner into the bore of the magnet! This statement is A. TRUE. B. FALSE. 23. NEVER lean against or push on the magnet! This statement is A. TRUE. B. FALSE. 24. On the PINMRF Varian spectrometers, the sample temperature controller should normally be off. This statement is A. TRUE. B. FALSE. 25. When leaving the spectrometer, you should A. click on exit and log out. B. click on exit. C. click on eject and terminate and then insert and lock standard sample. D. insert the lock sample, issue the standard command, exit VNMR, then log out. 5
26. When is it acceptable for you to take metal objects near the magnet? A. When there is no sample in the magnet. B. When there is no experiment running. C. When the metal objects are small, such as paper clips or staples. D. When no-one else is around. E. When you want to show your friends how powerful the magnet is. F. Never. 27. In case of an emergency, where are the contact names and phone numbers listed? A. On a bulletin board or other vertical surface readily seen when sitting at the computer. B. On the desk by the phone. C. On the desk by the computer. D. On the wall directly above the computer. 28. Which NMR experiments in the list below require additional training from PINMRF staff before you are allowed to run them on the PINMRF Varian spectrometers? A. Low-temperature VT. B. The 13 C DEPT experiment. C. Any experiment involving nuclei other than 1 H, 19 F, 31 P, 13 C or 11 B (300-2, 4100 BRWN). D. A and C. 29. If your NMR tube fits very loosely in the sample spinner, what should you do? A. Stick a piece of Kimwipe or other paper into the spinner to help hold the sample. B. Remove the rubber o-ring from the spinner. C. Put your NMR sample into a new tube that fits correctly. D. Check the fit of the lock standard sample if it is loose too, notify PINRMF staff. 30. If your lock signal is unstable (it fluctuates to higher and lower levels) what should you do to correct this? A. Stop your work immediately and notify PINMRF staff. B. Eject your sample and restart the process again. C. Decrease the lock gain. D. Decrease the lock power. 6
31. Which 2D NMR experiment(s) are set up for you to run on the Varian Inova-300 s using standard parameter sets? A. COSY. B. TOCSY. C. HMQC / HMBC. D. HETCOR. E. All of the above. F. A and D. 32. If an NMR magnet quenches while you are running an experiment, what should you do? A. Run out of the lab as fast as you can and then set off the nearest fire alarm. B. Stop your experiment and remove your sample from the magnet, then notify PINMRF staff. C. Open the lab windows and doors, cover the magnet, then notify PINMRF staff. D. Leave the lab immediately, crawling close to the floor if necessary, then notify PINMRF staff. E. Notify PINMRF staff, then wait until the vapor clears to continue your experiment. F. Call 911 from the lab phone and then run out of the lab as fast as you can. 33. You are running a 1 H NMR experiment and you notice that the peaks in your spectrum are broad and have an extra splitting on the upfield side. Which of the following are possible source(s) of the problem? A. The shims are not optimized. B. The probe tuning is poor. C. You loaded the wrong parameter set. D. The sample is not spinning. E. The sample volume is too small. F. The sample temperature controller is set to 323K. 34. You are trying to print out your spectrum but the printer is not responding. Which of the following activities are reasonable for you to attempt regarding the problem? A. Check that the printer has paper if not, you may reload the printer. B. Execute the plot command approximately 20 times to force the printout to appear. C. Turn off the printer s power, wait one minute, then power up the printer again. D. Turn off the power to the spectrometer computer. E. Notify PINMRF staff. 7
35. Which of the following represents an ON-resonance deuterium lock signal for a single deuterated solvent with one deuterium resonance? A. D. B. E. C. F. A B C D E F 36. In order to monitor the sample temperature, which command will open the temperature control window? A. two (temperature window open) B. edtemp C. edte D. temp 8