easyspiral The world's fastest Spiral plater

easyspiral The world's fastest Spiral plater

Our quality for your lab Designer and manufacturer for microbiology R&D leadership for innovative and reliable products Worldwide distribution network in more than 85 countries Made in France interscience R&D center and manufacturing plant Mourjou FRANCE Spiral method: 35 years experience The Spiral method was designed in 1973 to automate the routine work of bacterial enumeration by Dr. Ed Campbell, researcher at the FDA (Food & Drug Administration). With François Jalenques, friend and founder of interscience, they patented an updated method in 1992. Since then Spiral automatic platers have been a reference for applications in food microbiology, medical bacteriology, research on food preservatives or cosmetological factors in compliance with the NF V08-100, NF V08-050, NF V08-034, ISO 7218, ISO 4833-2, ISO 15189, FDA-BAM and AOAC standards. interscience has proudly launched the 3 rd generation of Spiral platers with easyspiral and easyspiral Pro. François Jalenques & Dr. Ed Campbell Spiral DS First Spiral Plater 1979 Spiral DS+ Automatic Spiral Plater 1996 easyspiral /easyspiral Pro World fastest Spiral platers Worldwide patented 2010 2 30 chemin du Bois des Arpents 78860 St Nom FRANCE - T: +33 (0)1 34 62 62 61 - info@interscience.com - www.interscience.com

Disinfectant Sterile water What is Spiral method? With easyspiral automatic plater, increase your lab capacity with a counting range from 100 to 1x10 7 CFU/mL on one single Petri dish without prior dilution. Manual plating method Automatic Spiral method This method requires repetitive actions: at least 4 dilutions and 4 successive platings are necessary to obtain one good and readable Petri dish. dilution 1/10 spread 0.1 ml dilution 1/10 spread 0.1 ml dilution 1/10 spread 0.1 ml dilution 1/10 spread 0.1 ml With this method, make your analyses on 1 single Petri dish! easyspiral Pro + sample - sample All dilutions on 1 Petri dish! 1 st Petri dish 2 nd Petri dish 3 rd Petri dish 4 th Petri dish Incubation Incubation Incubation Incubation Incubation From 100 to 1x10 7 CFU/mL on 1 single Petri dish Up to 75% less consumables Full plating cycle in 25 seconds! How to count? With the Spiral method, a logarithmically decreasing volume of sample is dispensed on the surface of a rotating Petri dish in an Archimedes spiral. The volume is calibrated and known at every point of the Petri dish. Bacterial concentration is determined by dividing the number of colonies found by the volume dispensed in the same sector of the dish. For manual counting, count the colonies in the fi rst A or B outer segment and reach a minimum of 20 colonies. Repeat the same operation on the opposite side of the Petri dish. Manual colony counting Automatic colony counting Scan 100 with Spiral counting grid or Scan 300 Scan 500 Scan 1200 Scan 4000 30 chemin du Bois des Arpents 78860 St Nom FRANCE - T: +33 (0)1 34 62 62 61 - info@interscience.com - www.interscience.com 3

easyspiral revolutionary effi ciency The new easyspiral patented technology, exclusively developed by interscience, allows the automatic plating of your sample on a Petri dish in only 25 seconds with a decreasing concentration (counting range: from 100 to 1x10 7 CFU/mL). A completely new architecture with a revolutionary rotating arm allows a full cycle in 25 seconds (disinfection, sample-taking and plating). All the liquid fl ows are visible. No exterior maintenance is needed. easyspiral is equipped with an innovative unique stylus cleaning device, which guarantees a perfect cleaning and disinfection of the stylus, with no risk of cross-contamination. Autoclavable bottles Secure and easy connection by CPC Connectors 2 L bottles 600 cleaning cycles Auto disinfection The inside and outside of the stylus are cleaned by overfl ow technology No cross contamination PATENTED Sampling area Fast and accurate fi lling in a sterile beaker DB50, sterile and autoclavable Stainless steel housing Compact, 40 cm wide Can be used inside and outside a laminar fl ow 4 30 chemin du Bois des Arpents 78860 St Nom FRANCE - T: +33 (0)1 34 62 62 61 - info@interscience.com - www.interscience.com

easyspiral ensures fast sample platings with 99.5% repeatability and reproducibility, and reliable analyses by harmonizing the work of the whole lab team. Cleaning liquid sensor Alarm when the bottle is empty Rotating arm architecture Automatic full cycle: 25 seconds PATENTED Modular Petri dish stand Switch from 90 to 150 mm Petri dishes Intuitive control panel Easy to use No training needed Monthly cleaning 1 button to access the intensive, periodic cleaning cycle (10 mn approx.) NF V08-100 NF V08-050 XP V08-034 ISO 7218 ISO 4833-2 ISO 15189 FDA BAM Bacteriological Analytical Manual AOAC 977.27 30 chemin du Bois des Arpents 78860 St Nom FRANCE - T: +33 (0)1 34 62 62 61 - info@interscience.com - www.interscience.com 5

easyspiral technology Delivering the fastest cycle time ever, easyspiral has an impressive rotating arm architecture, new patented disinfection system avoiding risks of cross-contamination and full traceability of the operations with a counting range from 100 to 1x10 7 CFU/mL on 1 Petri dish. The fastest Spiral plater: only 25 seconds for a full cycle! 25 seconds for a full cycle (disinfection, sample-taking and plating) Rotating arm with high speed movement Patented electronic robot system 10 Petri dishes plated in less than 2 mn (with the same sample) Overflow technology* sample drains away cleaning with alcohol rinsing with sterile water No cross-contamination Unique patented disinfection system by overfl ow 600 cleanings without changing bottles (2L) USB programmable volume & traceability** Easy PC connectivity via USB From 10 to 1000 microliters programmable volumes 3 preset plating modes Monitoring software with traceability XLS Excel USB Consumables reduced up to 75%! Reduced budget for consumables No specifi c consumables needed More space in your laboratory *Scientifi c study available upon request **On easyspiral Pro 6 30 chemin du Bois des Arpents 78860 St Nom FRANCE - T: +33 (0)1 34 62 62 61 - info@interscience.com - www.interscience.com

Plate & Count System + datalink Plate & Count System + datalinktm offer quick and reliable results from automatic plating to colony counting with total traceability. GREAT SAVINGS: Up to 75 % savings in time, consumables and space QUICK: Full plating cycle in 25 seconds and counting in 1 click. No need of manual data input as the Scan colony counter retrieves it and adjusts automatically. RELIABLE: Repeatable and reproducible results up to 98 % FULL TRACEABILITY WITH Automatic saving of data and results datalinktm: easyspiral Pro and Scan 4000 How does it work? STEP 1 Plate with easyspiral Pro. easyspiral software collects the plating data. al Pro easyspir STEP 2 Print the label with Datamatrix code. Stick the label on the plated Petri dish and place in the incubator. 24-72 h incubation AUTO PDF JPG JPEG Scan 4000 Adobe XLS Excel LIMS STEP 3 Once the colonies have grown, scan the Datamatrix code. The Scan colony counter automatically adjusts its settings thanks to the Datamatrix label s data. Click on COUNT. Export the data. * Please check LIMS compatibility D DOWNLOAD S C SOFTWARE SCAN www.interscience.com 30 chemin du Bois des Arpents 78860 St Nom FRANCE - T: +33 (0)1 34 62 62 61 - info@interscience.com - www.interscience.com 7

Technical specifi cations easyspiral easyspiral Pro Reference 412 000 413 000 Syringe capacity 1000 µl Preset deposited volume 50 or 100 µl 50, 100 or 200 µl Counting range 300 to 1.3 x 10 5 CFU/mL 100 to 1 x 10 7 CFU/mL Plating time (50 µl - disinfection + plating) 25 seconds Overflow technology 8 bars pressure Disinfectant autonomy (in normal mode) 1000 cycles (2L bottles) Distilled water autonomy (in normal mode) 600 cycles (2L bottles) Successive plating capacity with the same sample Up to 20 Petri dishes (50 µl) Automatic disinfection program 90 mm Petri dish 150 mm Petri dish - Exponential deposition mode Circle deposition mode Constant deposition mode - Excel export traceability - Programmable deposited volume (via USB) Dimensions Weight Power supply Accessories Housing protection for platers Transparent housing for working outside laminar fl ow Ref 413 001 Your local distributor datalink Traceability system Ref 410 100 - from 10 to 1000 µl 40 x 41.5 x 29 cm 16.4 Kg 100-240 V~ 50-60 Hz www.interscience.com DB 50 beakers 1 000 sterile beakers for Spiral platers Ref 415 100 APPLICATIONS Microbiological analyses Food quality control Bacterial cinetics MIC of antibiotics Challenge test: cosmetics control Pharmaceutical control Delivered with: 1 blue dye for testing, 1 syringe with silicone tubing, Spiral counting grids: 90 mm & 150 mm*, support test container, 2 fi lters, 1 replacement stylus, 4 connector sets for GL45 bottles, 1 GL45 double connection connector set, 1 pot of EnzyClear, 1000 sterile beakers, power cord, user manual, 150 mm Petri dish ring*, software on a CD-ROM*, USB cable*. *Only for easyspiral Pro Bar-code reader Barcode reader for traceability Ref 522 000 INTERNATIONAL 30, chemin du Bois des Arpents - 78860 St Nom - FRANCE T: +33 (0)1 34 62 62 61 - F: +33 (0)1 34 62 43 03 - Email: info@interscience.com USA & CANADA 32 Cummings Park - Woburn, MA 01801 - USA T: +1 781 937 0007 - F: +1 781 937 017 - Email: sales.usa@interscience.com CHINA 277 588-200030 : +86 (0)21-64739390 - +86 189 3097 0733 - : sales.china@interscience.com SOUTH-EAST ASIA 541 Orchard road - 09-01 Liat Towers - SINGAPORE 238881 T: +65 6933 1389 - +65 8549 1217 - Email: sales.asia@interscience.com 3073 easyspiral_b_0216. Pictures and information in this brochure are not contractually binding. Spiral, easyspiral, easyspiral Pro, Spiral Plater, Plate & Count System, Scan are registered trademarks. INTERSCIENCE reserves the right to change or improve the specifi cations of its products without notice. Please visit www.interscience.com for current updates and additional information. RCS 950 356 220 Versailles. INTERSCIENCE SARL F78860. EN

easyspiral Dilute The world's fi rst automatic diluter & plater A revolution in serial dilutions!

Our quality for your lab Designer and manufacturer for microbiology R&D leadership for innovative and reliable products Worldwide distribution network in more than 85 countries Made in France interscience R&D center and manufacturing plant Mourjou FRANCE Spiral method: 35 years experience The Spiral method was designed in 1973 to automate the routine work of bacterial enumeration by Dr. Ed Campbell, researcher at the FDA (Food & Drug Administration). With François Jalenques, friend and founder of interscience, they patented an updated method in 1992. Since then Spiral automatic platers have been a reference for applications in food microbiology, medical bacteriology, research on food preservatives or cosmetological factors in compliance with the NF V08-050, NF V08-034, ISO 7218, ISO 4833-2, ISO 15189, FDA BAM and AOAC 977.27 standards. Today interscience is proud to launch the 4 th generation of Spiral platers with easyspiral Dilute : the world's fi rst automatic diluter & plater. F. Jalenques & Dr. Ed Campbell 1979 Spiral DS First Spiral plater 1996 Spiral DS+ Automatic Spiral plater 2010 easyspiral World's fastest Spiral plater PATENTED 2012 easyspiral Dilute World's fi rst automatic diluter & plater PATENTED

interscience is proud to introduce easyspiral Dilute, a 2-in-1 automatic diluter and plater, which allows you to do 5 x 1/10 th serial dilutions. It will then automatically plate on 1 single Petri dish, with a countable range from 30 to 1 x 10 12 countable CFU/mL. No more manual dilutions and plating is automatic! Place sample 2 Plate 1 Dilute Place your sample in the beaker 1. The easyspiral Dilute will automatically dilute the sample up to 5 times 2. The easyspiral Dilute will automatically plate the sample on a Petri dish up to 1 x 10 12 countable CFU/mL Why use an automatic diluter & plater? easyspiral Dilute allows you to save 50% on consumables and 50% on your time. Comparison between manual and automatic method below. Example of 50 samples: Dilution: 10-7 Plating: 10-5, 10-6 and 10-7 Manual method CONSUMABLES Dilution 400 tubes Automatic method CONSUMABLES Dilution 350 beakers 350 pipet tips Plating Plating 450 Petri dishes 150 Petri dishes TIME 10h10 TIME 5h50 3

Key features Compact 38 cm wide, can be used inside and outside a laminar fl ow Stainless steel housing High precision glass syringe Typical accuracy of 0.5 % 4x more precise than a basic pipet No more pipet calibration High speed automation Fast rotating arm 1 disinfection, 5 dilutions and 1 plating in 134 s PATENTED Automatic disinfection Overfl ow technology cleaning system No cross contamination PATENTED One-touch plating Choose from 4 plating modes Plate on 55, 90 and 150 mm Petri dishes Easy serial dilutions Choose your dilution factor Do 10-5 dilution or more automatically No more 9 ml tube preparation PATENTED High capacity 2 fully autoclavable bottles (disinfectant & diluent) and connectors 4

How does it work? 1. Dilution cycle 1 sample Sample intake 2 sample 10-1 0.5 ml of sample is deposited 3 sample 10-1 4.5 ml of diluent is added to the sample and mixed The above dilution cycle is repeated for the next serial dilution. 1 sample 10-1 0.5 ml of 10-1 diluted sample intake 2 sample 10-1 10-2 0.5 ml of 10-1 diluted sample is deposited Dilution cycle can be repeated as many times as you wish. 2. Plating cycle Examples: Constant mode Homogenous surface deposition 50 µl, 100 µl, 200 µl and USB programmable Exponential mode Decreasing surface concentration 50 µl, 100 µl, 200 µl and USB programmable Circle mode 3 or 6 surface dilutions on 1 Petri dish 10 µl inner circle, 16 µl middle circle and 25 µl outer circle Pour mode Homogenous deposition* in pour plate mode 50 µl, 100 µl, 200 µl and USB programmable Before incubation Homogenous surface deposition + concentrated - concentrated 10-1 10-2 10-3 Homogenous deposition in pour plate mode* After incubation Easy colony counting: No colonies on the edge of the Petri dish Counting on Petri dish up to 1 x 10 12 CFU/mL Savings on Petri dishes Easy colony counting for invasive colonies. Pharmacopoeia compliant * In pour mode, easyspiral Dilute deposits one sample drop at the bottom of an empty Petri dish. Then, the user adds the molten agar on top and homogenizes it with the sample. How to count? Manual colony counting Automatic colony counting Scan 100 with Spiral counting grid or Scan 300 Scan 500 Scan 1200 Scan 4000 5

Questions What is special about the easyspiral Dilute? easyspiral Dilute is the only 2-in-1 equipment on the market making automatic serial dilutions and standardized plating. The accuracy of easyspiral Dilute is high: 0.5%. How can I check it? I usually dilute 1 ml to 1/10 and the easyspiral Dilute uses 0.5 ml to 1/10. Is easyspiral Dilute as precise with a volume divided by 2? It is easy and automatic. Test of the deposited volume during plating: Press on the plating volume test button. This function will fi ll a previously weighed beaker with 500 µl. Weigh the distributed volume to check the results. Test of the volume of the dilutions: Press on the dilution volumes test button. This function will fi ll 2 previously weighed beakers: one with 0.5 ml and the 2 nd one with 4.5 ml. Weigh the distributed volumes to check the results. Usually the average accuracy for the 9 ml tubes fi lling and 1 ml pipetting is 2% with the need to calibrate and regularly check the pipets you are using. easyspiral Dilute has an average accuracy of 0.5% on 0.5 ml and 4.5 ml volumes because it works with a high precision Hamilton TM glass syringe in a liquid displacement circuit. With volumes divided by 2, precision and repeatability are then much higher! Which diluents can I use? easyspiral Dilute works with saline solutions (e.g.: Ringer's solution, physiological saline). Avoid using diluents with nutrients as they may cause contaminations. Bacteria only remain a few seconds in the beakers before being deposited on the Petri dish so using a saline diluent has no effect on the fi nal enumeration. It is however possible to use nutrient solutions as diluent in the semi-automatic "My Diluent" mode. In that case, fi ll in the beakers with 4.5 ml diluent before processing. I need to make a 10-7 dilution. How can I do it? The easyspiral Dilute can do as many dilutions as you wish. Do a 10-5 dilution, take the last beaker and place it as the mother sample and then proceed with a new automatic dilution sequence. 6

Accessories & technical specifi cations Housing protection datalink TM Beakers DB 50 Barcode reader Transparent housing for working outside laminar fl ow. Traceability system 1 000 sterile beakers for Spiral platers (5 ml) Barcode reader for traceability Ref. 413 001 Ref. 410 100 Ref. 415 100 Ref. 522 000 easyspiral Dilute is delivered with 1 000 sterile beakers, 1 blue dye for tests, 1 box of EnzyClear, 4 sets of GL 45 bottle connectors, Petri dish rings: 55, 90 & 150 mm, Spiral counting grids: 90 & 150 mm, circle counting grids: 90 & 150 mm, power cord, user s manual, monitoring CD-ROM software, USB cable. easyspiral Dilute Reference 414 000 Petri dish diameter 55 mm, 90 mm and 150 mm Syringe capacity 1 000 µl Programmable dispensed volume (via USB) from 10 µl to 1 000 µl Preset dispensed volume 50 µl, 100 µl and 200 µl Detection in CFU/mL of Spiral plating 30 to 1 x 10 12 CFU/mL 55 mm: constant, pour plate Plating modes 90 mm: exponential (10-5 ), constant, circle (3 dilutions), pour plate 150 mm: exponential (10-7 ), constant, circle (6 dilutions), pour plate Number of dilutions at 1/10 th for 1 cycle 5 1 disinfection + 5 dilutions + 1 plating of 1 dilution 134 s 1 disinfection + 5 dilutions + 1 plating of each dilution 234 s Disinfectant autonomy 1000 (with 2 L bottles) Diluent autonomy 63 cycles (with 2 L bottles) Circle mode (patented) 3 dilutions on a 90 mm Petri dish 6 dilutions on a 150 mm Petri dish Stylus disinfection system (patented) inside and outside by Overfl ow technology Pressure in the stylus 8 bars Successive plating capacity with the same sample up to 20 Petri dishes (50 µl) Traceability excel TM, datamatrix labels Monthly cleaning program Filling time programmable via USB My Diluent key Sample mixing before dilution/plating Controlled by microprocessor Dimensions (w x d x h) 39 x 42 x 29 cm Weight 15.6 kg Power supply 100-240 V~ / 50 Hz to 60 Hz Power 65 W 1 year guarantee (after registration) All stainless steel Made in France NF V08-050 NF V08-034 ISO 7218 ISO 4833-2 ISO FDA BAM Bacteriological Analytical AOAC ISO 9001 WEEE 2002/96/EC RoHS 2002/95/EC 15189 Manual 977.27 MADE IN 2008 FRANCE 7

Distributor INTERNATIONAL 30, ch. Bois Arpents - 78860 St Nom - FRANCE T : +33 (0)1 34 62 62 61 - F : +33 (0)1 34 62 43 03 info@interscience.fr - www.interscience.fr USA & CANADA 199 Weymouth ST. - ROCKLAND - MA 02370 - USA T: +1 781 792 2133 - F: +1 781 792 2134 info@intersciencelab.com - www.intersciencelab.com CHINA 277 718-200030 : +86 (0)21-64739390 - : +86 189 3097 0733 sales.china@interscience.fr - www.interscience.fr/china www.interscience.fr easyspiral Dilute_B_0615. Pictures and information in this brochure are not contractually binding. Spiral, easyspiral, easyspiral Dilute, Scan are trademarks. This document is the translation of the french brochure and is not contractually binding. INTERSCIENCE reserves the right to change or improve the specifi cations of its products without notice. Please visit www.interscience.fr for current updates and additional information. RCS 950 356 220 Versailles. INTERSCIENCE SARL F78860. EN

Appendix 2. easyspiral / easyspiral Pro validation studies 1. Spiral plating method validation Purpose To study the reliability of Spiral plating with easyspiral comparing to the reference method (classical surface plating). Equipment and methods Five bacterial strains (three gram+ and two gram-) are tested: Lactobacillus casei var. rhamnosus, Bacillus cereus (ATCC 11778), Bacillus subtilis (ATCC 6633), Escherichia coli (ATCC 8739) and Pseudomonas aeruginosa (ATTC 9027). Bacterial cultures are diluted in buffered peptone water then plated on Petri dish agar (90 mm), using classical method and Spiral method (50!L in exponential mode). After 48h of incubation at 37 C (±1) on MRS (De Man, Rogosa, Sharpe) for Lactobacillus casei and 18h (±2) at 37C (±1) on PCA (Plate Count Agar) for Bacillus cereus, Bacillus subtilis, Pseudomonas aeruginosa and on Macconkey for Escherichia coli, counting is made with an automatic colony counter (Scan 1200). Results analysis Counting results are expressed in Log CFU/mL (Colony Forming Unit) and a regression analysis is made to assess the correlation between, Spiral method with easyspiral Pro and classical plating method. Results - Bacillus cereus: Spiral method (Log CFU/mL) 5 4.5 4 3.5 3 y = 0,974x + 0,0217 R2 = 0,982 2.5 2.5 3 3.5 4 4.5 5 Classical method (Log CFU/mL) Figure 1: Correlation between Bacillus cereus counting (Log CFU/mL) obtained by plating in classical and Spiral method. 85/125 easyspiral / easyspiral Pro : Guide d installation et d utilisation V3

- Bacillus subtilis: 5 Spiral method (Log CFU/mL) 4.5 4 3.5 3 y = 0.9923x + 0.0238 R 2 = 0.9853 2.5 2.5 3 3.5 4 4.5 5 Classical method (Log CFU/mL) Figure 2: Correlation between Bacillus subtilis counting (en Log CFU/mL) obtained by plating in classical and Spiral method. - Lactobacillus casei var. rhamnosus: 5.5 Spiral method (Log CFU/mL) 5 4.5 4 3.5 y = 0,9036x + 0,335 R 2 = 0,994 3 3 3.5 4 4.5 5 5.5 Classical method (Log CFU/mL) Figure 3: Correlation between Lactobacillus casei var. rhamnosus counting (en Log CFU/mL) obtained by plating in classical and Spiral method. easyspiral / easyspiral Pro: Installation guide and user s manual V3 86/125

- Escherichia coli: 5.5 Spiral method (Log CFU/mL) 5 4.5 4 3.5 y = 0,9822x + 0,0853 R 2 = 0,989 3 3 3.5 4 4.5 5 5.5 Classical method (Log CFU/mL) Figure 4: Correlation between Escherichia coli counting (en Log CFU/mL) obtained by plating in classical and Spiral method. - Pseudomonas aeruginosa: Spiral method (Log CFU/mL) 5 4.5 4 3.5 3 2.5 y = 0,9632x + 0,1039 R 2 = 0,990 2 2 2.5 3 3.5 4 4.5 5 Classical method (Log CFU/mL) Figure 5: Correlation between Pseudomonas aeruginosa counting (en Log CFU/mL) obtained by plating in classic and easyspiral pro method. Conclusion Results show a strong correlation (R 2 " 0.982) between Spiral method with easyspiral Pro and Classical method. Hence the average difference of CFU/mL log between the two methods is resulting to be not significatif 12 times lower than the maximal deviation (0.5 log) authorised by AFNOR NF V08/100. 87/125 easyspiral / easyspiral Pro : Guide d installation et d utilisation V3