High-speed, high-resolution digital slide scanner with network features

Transcription

1 High-speed, high-resolution digital slide scanner with network features R

2 NanoZoomer 2.0 series proposes new methods in drug development and medical research. The NanoZoomer 2.0 series is a system that converts glass slides into digital slides by scanning them quickly at high resolutions. Digital slides can be stored as high-definition, high-quality digital image Tissue slide (26 mm 76 mm) data in which you can zoom in and out on any portion of the entire sample with a simple mouse operation, as if you were operating a microscope. In addition, the Z-stack multi-level slide scanning feature allows you to view thick samples by changing the focus. Zoom The NanoZoomer 2.0 series scans glass slides and converts it into digital slides quickly. The zoom function of the NanoZoomer 2.0 series allows viewing magnified sections in detail and with high resolution. Digital slides have many advantages! Digitizing slides opens up a variety of new possibilities. Copying and Sharing Digitized slides can be copied and shared. This feature of digital slides can be used in a variety of applications. For example, a large group of people can observe and discuss a single sample. Slide Storage Digital data does not deteriorate, and it is more secure from damages and losses than glass slides. You can view digital data in its original quality anytime and anywhere. Networks Databases Using the Internet or a local area network, you can observe and evaluate slides from a distant location. Large numbers of digital slides can be stored into a database and incorporated into a laboratory information system. You can share data and construct slide libraries with distant facilities and research institutes. * The NanoZoomer 2.0 series as medical devices may be subject to government regulations where they are used. Hamamatsu makes no representation with regard to the conformity of these products to these regulations. Please consult your local Hamamatsu representative for more information. 2

3 High-speed, high-resolution digital slide scanner with network features Feature Process up to 210 slides automatically The NanoZoomer 2.0-HT processes up to max. 210 slides automatically using its dedicated slide cassettes. You can save time by processing large amounts of samples overnight. The NanoZoomer 2.0-HT can also automatically read a slide s barcode information and use it to name the slide file. Feature Automatically process up to 6 slides of regular size or 2 slides of double size The NanoZoomer 2.0-RS is compact and affordable. It maintains the performance of the NanoZoomer 2.0-HT except for the number of slides processed automatically. It processes up to 6 slides of regular size (76 mm 26 mm) or 2 slides of double size (76 mm 52 mm) automatically. (The processing of double size slides is an optional feature.) Functions High-speed scanning at approximately 1 min. 40 s. for 1.9 billion-pixel slide image* The NanoZoomer 2.0 series achieves both high speed and high sensitivity by using TDI line scanning instead of traditional CCD tiling. A single slide can be scanned at a resolution of approx. 1.9 billion pixels in approx. 1 minute 40 seconds. * 1.9 billion pixels is based on the sample size of a 20 mm 20 mm at 20 mode scan. Additional 1 minute is necessary for setting up a slide. Improved 40 mode scanning speed, ideal for routine scans The NanoZoomer 2.0 series reduces scanning time in 40 scan mode to 4.5 minutes only. Ability to observe fluorescent samples provides a new approach to fluorescence imaging By using the Fluorescence Illumination Optics L10387 with the NanoZoomer 2.0 series, you can observe and analyze fluorescent samples without worrying about photobleaching. It will open up new applications which could not be done with a traditional microscope. Optional image distribution software The NanoZoomer 2.0 series has optional image distribution software that is intended to facilitate the broader use of digital slides. You can use the free slide viewing software to view the images on a server at any time anywhere. Z-stack feature for thick samples There are samples which have 3D structures such as clumps of cells and thick tissues. They require focus adjustment during observation. To handle these kinds of slides, the NanoZoomer 2.0 series is equipped with the Z-stack feature that allows you to focus on different depths in the sample. The NDP.view viewer software lets you adjust the focus on a Z-stack slide much like you would adjust the focus of a microscope. You can also point to an area of interest and let NDP.view apply autofocus for maximum clarity. Depth direction μm μm μm μm μm ±0.00 μm 0.50 μm 1.00 μm 1.50 μm 2.00 μm 2.50 μm -2.5 μm X-axis Z-axis -1.5 μm Y-axis +2.5 μm +1.5 μm ±0.0 μm 3

4 TDI (Time Delay Integration) technology enables the quick production of high-resolution digital slides. The NanoZoomer series scanning unit uses ''line scanning method'' and ''TDI method.'' It can convert a large number of glass slides into digital slides automatically in a short time. Features Variety of superior scan features from the expertise of Hamamatsu sensor technology. TDI combines both high speed and high sensitivity The NanoZoomer 2.0 series uses time delay integration (TDI) to provide high speed and high sensitivity simultaneously. Hamamatsu technology allows synchronizing TDI sensor signal with the movement of a microscopic sample object being scanned. The key to improved scanning The NanoZoomer 2.0 series achieves both high speed and high sensitivity by using a unique 3-chip TDI line scanning method instead of traditional CCD tiling. Line scanning makes scanning faster Traditional CCD tiling High-speed scanning at approximately 1 min 40 s. for 1.9 billion pixel slide image* A 1.9 billion pixels digital slide is created at 20 mode in approx. 1 minute 40 seconds. It also creates a 7.6 billion pixel digital slide at 40 mode in approx. 4.5 minutes. * 1.9 billion pixels is based on the sample size of a 20 mm 20 mm at 20 mode scan. Additional 1 minute is necessary for setting up a slide. TDI Line Scanning Image composition Relatively slow scan and 2D composition of tiles Dedicated system design makes the NanoZoomer 2.0 series user friendly and highly reliable. The NanoZoomer 2.0 series is a specialized machine designed for routine use. It is user friendly and highly reliable. It employs a variety of sensors monitoring mechanical operations to ensure the safety of your slides. Image composition The image is scanned line by line. Fast scanning and 1D composition 3-chip TDI camera provides superior color reproduction The NanoZoomer 2.0 series uses a 3-chip TDI camera to accurately reproduce sample colors. It enables observing minute variations in the colors of the sample. The 3-chip TDI camera has red, green and blue channels, and they are used to produce a single RGB image. Unique TDI sensors realizes both high sensitivity and high speed simultaneously Line scanning Sensor The signal comes out without integration. Automatic processing of a large number of slides The NanoZoomer 2.0-HT processes up to max. 210 slides automatically using its dedicated slide cassettes. Just load the slides and press the start button to automatically load and scan each sample. The NanoZoomer 2.0-RS is compact and affordable. It maintains the performance of the NanoZoomer 2.0-HT except for the number of slides processed automatically. The arrows show the movement of the sample. TDI sensor: Four steps (the actual NanoZoomer 2.0 sensors have 64 steps) The scanned object moves constantly, and the signal is reinforced at each stage by integrating the signal. This leads to fast and sensitive scanning. TDI sensor (Four steps) Signal level 1st stage 2nd stage 3rd stage 4th stage 4

5 Quickly converts transparencies into high-resolution digital slides! Examples A powerful tool for a wide range of applications in drug development and medical research Application for ES and ips cells research It is used to test differentiation activity of ES/iPS cells by injecting into an immunodeficient mouse. From any part of a larger overall picture, several differentiated tissues are observed. ES/iPS cells 3 mm 250 μm An immunodeficient mouse Teratoma formation by using mouse ips cells (HE-stain). (Entire image) Epithelial-like tissues are observed. (Selected area, magnified) Courtesy of Center for ips Research and Application, Kyoto University Observing H&E stained samples H&E staining is the popular staining method for tissue samples. Stomach cancer H&E stain Courtesy of Hiroshi Ogawa MD, Department of Pathology, Seirei Mikatahara Hospital. Pancreatic AVM with anisakiasis, resulting in pancreatic bleeding Courtesy of KobeCity Medical Center General Hospital, Department of Pathology, Yukihiro Imai, MD Ph.D. Conferences using digital slides With digital slides, you can share the same sample among many people without worrying about sample deterioration. Expressed parvalbumin mrna (In situ hybridization method) Evaluation of tumor and virus infection by gene expression in a cell or a tissue. PAS stained kidney biopsy sample Courtesy of Department of Pathology, The University of Tokyo Hospital, Hiroshi Uozaki, M.D., Ph.D. NBT and BCIP developed color of mouse brain tissue slice Courtesy of Ms. Chihiro Saruta, Teiichi Furuichi PhD, Laboratory for Molecular Neurogenesis, Riken Brain Science Institute. 5

6 Options Optional fluorescence sample scanning feature with Fluorescence Imaging Module Digitization of fluorescence samples enables long-term observation without worrying about photobleaching. The Fluorescence Imaging Module combined with the NanoZoomer 2.0 series digitizes entire fluorescence-stained slides with high speed and high resolution. The digitized slides are saved as digital data, and this enables longterm microscopic observation without photobleaching. The L scans fluorescence slides with single, double and triple stains by manually replacing the filter cube. The L and -22, which feature excitation and fluorescence filter wheels with 6 filters each, scan fluorescence slides sequentially by automatically switching filters. The L11600 provides a new approach to fluorescence imaging! The Fluorescence Imaging Module enables scanning various fluorescence slides. Scan multiple fluorescence probes The L11600 with NanoZoomer 2.0 series scans and digitizes slides stained with multiple fluorescence probes such as Q-dots, fluorochromes, fluorescence proteins, and others. The filter wheel unit enables continuous, automatic scanning with 6 switchable filters for fluorescence and excitation wavelengths. Multiple images are acquired sequentially. Fluorescence Imaging Module L11600 Superimpose images of entire tissues (samples) The viewer software can superimpose a bright field image and a fluorescence image, or two or more fluorescence images. It allows observation of protein localization and expression in the entire image at any magnification. High-power and long-life light source without alignment Newly designed fluorescence excitation light source LX2000 realizes high power and long life. It does not require optional alignment even when a lamp is replaced. You can zoom in on any part of an entire fluorescent sample. Features dark field illumination for sample identification Fluorescent sample locations are difficult to determine using bright field illumination. So the NanoZoomer 2.0 series uses dark field illumination* to determine sample locations. This makes locating and scanning samples easier. * Under patent filing. Features highly efficient optics created for use with a TDI sensor The Fluorescence Imaging Module allows the NanoZoomer 2.0 series to scan even weak fluorescent samples quickly. It also prevents unnecessary photobleaching by minimizing the area of excitation. 6

7 Quickly converts fluorescent samples into high-resolution digital slides! Examples A powerful tool for a wide range of applications in drug development and medical research Rapid and High-Fidelity Imaging of Fluorescence-Labeled Q-dots Human pancreas Nucleus: Hoechst Chromogranin (endocrine gland, islets of Langerhans): Qdot655 Cytokeratin (exocrine gland): Qdot565 Courtesy of the Department of Pathology, Keio University School of Medicine Horizontal Section of the Nile Rat Brain Showing the Crossing of Retinal Axons in the Optic Chiasm Left eye was injected with Cholera Toxin B conjugated to Alexa 488; Right eye with Cholera Toxin B conjugated to Alexa 596. Images show the axon bundles as they cross to the opposite sides of the brain. Courtesy of the Harvey Karten, University of California-San Diego, USA; Yves Sauve, University of Alberta, Canada; Frederic Gaillard, Universite de Poitiers, Poitiers, France 7

8 Specifications NanoZoomer 2.0 series scanner Compatible glass slide Slide loader Scanning range Objective lens Scanning resolution Scanning method Barcode reader Scanning speeds 20 mode Image compression Slide format Power supply voltage Power consumption 40 mode NanoZoomer 2.0-HT (C ) 76 mm 26 mm, thickness 0.9 mm to 1.2 mm Automatic, max. 210 slides 25 mm 52 mm * When using A (optional slide tray for scanning double-size glass slides) Fluorescence Imaging Module L11600/L10387 L * for NanoZoomer 2.0-HT Light source** LX W Ultrahigh-pressure mercury lamp Number of filter cube position 1 Filter wheel 6Ex/6Em switching L * for NanoZoomer 2.0-RS Light source** Number of filter cube position Filter wheel LX W Ultrahigh-pressure mercury lamp 2 (Possible to switch cubes automatically) 6Ex/6Em switching *Filter cubes and filters are not included. For more information, please consult your local sales representative. **Size: 180 mm(w) x 299 mm(d) x 227 mm(h), Approx.6.8 kg, Power consumption: 400 V A NanoZoomer 2.0-RS (C ) 76 mm 26 mm / 76 mm 52 mm*, thickness 0.9 mm to 1.2 mm Standard size: 6 slides, Double size: 2 slides 25 mm 52 mm / 50 mm 52 mm 20 N.A μm/pixel (20 standard mode) and 0.23 μm/pixel (40 high resolution mode) TDI (Time Delay Integration) One-dimensional, standard (Option: two-dimensional) Approx. 1 min. (area: 15 mm 15 mm) Approx. 1 min 40 s. (area: 20 mm 20 mm) Approx. 2.5 min. (area: 15 mm 15 mm) Approx. 4.5 min. (area: 20 mm 20 mm) JPEG compression JPEG compressed image + slide information AC 100 V to AC 240 V 400 V A L for NanoZoomer 2.0-HT and -RS Light source** Filter cube Number of filter cube position Filter wheel LX W Ultrahigh-pressure mercury lamp 3 filter cubes: B excitation, G excitation, Triple bands (UV, B, G) 1 - Dimensional outlines (Unit: mm) NanoZoomer 2.0-HT Scanner C (with L11600) NanoZoomer 2.0-RS Scanner C (with L11600) ±5 319± ±5 637±5 308±5 860±10 235± ± ±5 540±5 230± ±5 5.4 * The NanoZoomer 2.0 series as medical devices may be subject to government regulations where they are used. Hamamatsu makes no representation with regard to the conformity of these products to these regulations. Please consult your local sales representative for more information. NanoZoomer is a registered trademark of Hamamatsu Photonics K.K.. Product and software package names noted in this documentation are trademarks or registered trademarks of their respective manufacturers. Subject to local technical requirements and regulations. Availability of products included in this promotional material may vary. Please consult with your local sales representative. Information furnished by HAMAMATSU is believed to be reliable. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications and external appearance are subject to change without notice Hamamatsu Photonics K.K. Homepage HAMAMATSU PHOTONICS K.K., Systems Division 812 Joko-cho, Higashi-ku, Hamamatsu City, , Japan, Telephone: (81) , Fax: (81) , export@sys.hpk.co.jp U.S.A. and Canada: Hamamatsu Corporation: 360 Foothill Road, Bridgewater, N.J , U.S.A., Telephone: (1) , Fax: (1) , usa@hamamatsu.com Germany: Hamamatsu Photonics Deutschland GmbH: Arzbergerstr. 10, D Herrsching am Ammersee, Germany, Telephone: (49) , Fax: (49) , info@hamamatsu.de France: Hamamatsu Photonics France S.A.R.L.: 19, Rue du Saule Trapu, Parc du Moulin de Massy, Massy Cedex, France, Telephone: (33) , Fax: (33) , infos@hamamatsu.fr United Kingdom: Hamamatsu Photonics UK Limited: 2 Howard Court, 10 Tewin Road, Welwyn Garden City, Hertfordshire, AL7 1BW, U.K., Telephone: (44) , Fax: (44) , info@hamamatsu.co.uk North Europe: Hamamatsu Photonics Norden AB: Smidesvägen 12, SE Solna, Sweden, Telephone: (46) , Fax: (46) , info@hamamatsu.se - Italy: Hamamatsu Photonics Italia S.R.L.: Strada della Moia, 1 int Arese (Milano), Italy, Telephone: (39) , Fax: (39) , info@hamamatsu.it Cat. No. SBIS0043E03 DEC/2010 HPK Created in Japan