GC/MS BATCH NUMBER: TK0106

Transcription

1 GC/MS BATCH NUMBER: TK0106 ESSENTIAL OIL: TURMERIC C02 ORGANIC BOTANICAL NAME: CURCUMA LONGA ORIGIN: INDONESIA KEY CONSTITUENTS PRESENT IN THIS BATCH OF TUMERIC C02 ORGANIC OIL % α-turmerone 25.4 ar-turmerone 20.0 β-turmerone 16.3 β-sesquiphellandrene 2.0 α-zingiberene 2.0 ar-curcumene 1.9 α-phellandrene 1.3 Comments from Robert Tisserand: Rich, sweet, spicy-woody-rooty odor quality. Constituents are in expected amounts nd St S. Twin Falls, ID * * planttherapy.com facebook.com/planttherapy * planttherapy.com/blog

2 Date : March 15, 2018 CERTIFICATE OF ANALYSIS - GC PROFILING SAMPLE IDENTIFICATION Internal code : 18C14-PTH1-1-CC Customer identification : Tumeric Organic CO2 - Indonesia - TK010671R Type : CO2 extract Source : Curcuma longa Customer : Plant Therapy ANALYSIS Method: PC-PA J19 - Analysis of the composition of an essential oil, or other volatile liquid, by FAST GC-FID (in French); identifications validated by GC-MS. Analyst : Sylvain Mercier, M. Sc., Chimiste Analysis date : March 15, 2018 Checked and approved by : Alexis St-Gelais, M. Sc., chimiste Note: This report may not be published, including online, without the written consent from Laboratoire PhytoChemia. This report is digitally signed, it is only considered valid if the digital signature is intact. Page 1/13

3 PHYSICOCHEMICAL DATA Physical aspect: Bright yellow liquid Refractive index: ± (20 C) CONCLUSION No adulterant, contaminant or diluent has been detected using this method. Page 2/13

4 ANALYSIS SUMMARY Identification DB-5 (%) DB-WAX (%) Classe Toluene * Simple phenolic α-thujene tr [0.01]* Monoterpene α-pinene Monoterpene Camphene 0.01* 0.01 Monoterpene α-fenchene [0.01]* tr Monoterpene Sabinene tr tr Monoterpene 6-Methyl-5-hepten-2-one tr tr Aliphatic ketone Myrcene Monoterpene α-phellandrene Monoterpene Δ3-Carene Monoterpene α-terpinene Monoterpene para-cymene Monoterpene Limonene 0.85* 0.12 Monoterpene 1,8-Cineole [0.85]* 0.78 Monoterpenic ether (Z)-β-Ocimene tr tr Monoterpene (E)-β-Ocimene tr 0.01 Monoterpene γ-terpinene Monoterpene Terpinolene Monoterpene Linalool Monoterpenic alcohol Unknown 0.01 tr Oxygenated monoterpene Unknown tr tr Oxygenated monoterpene cis-para-menth-2-en-1-ol Monoterpenic alcohol Camphor Monoterpenic ketone Unknown Oxygenated monoterpene Borneol * Monoterpenic alcohol δ-terpineol * Monoterpenic alcohol Terpinen-4-ol Monoterpenic alcohol 4-Methylacetophenone Simple phenolic para-cymen-8-ol Monoterpenic alcohol α-terpineol 0.07 [0.06]* Monoterpenic alcohol α-phellandrene epoxide Monoterpenic ether trans-piperitol * Monoterpenic alcohol Unknown Oxygenated monoterpene Unknown tr 0.08 Oxygenated monoterpene Carvotanacetone tr tr Monoterpenic ketone Unknown 0.03 Monoterpenic ester Thymol * Monoterpenic alcohol Carvacrol * Monoterpenic alcohol Unknown Unknown Unknown Unknown tr Unknown Unknown Terpene derivative Unknown Terpene derivative Unknown Terpene derivative Unknown Unknown Unknown Sesquiterpene Acora-3,7(14)-diene Sesquiterpene (Z?)-Vestitenone, or analog 0.01 tr Terpenic ketone Page 3/13

5 β-caryophyllene * Sesquiterpene α-santalene Sesquiterpene (trans?)-6-hydroxypiperitone Monoterpenic alcohol (cis?)-6-hydroxypiperitone * Monoterpenic alcohol γ-elemene Sesquiterpene trans-α-bergamotene 0.02 [0.41]* Sesquiterpene Unknown 0.28 Unknown (E)-Vestitenone Terpenic ketone α-humulene Sesquiterpene Unknown 0.01 [0.02]* Sesquiterpene (E)-β-Farnesene Sesquiterpene β-acoradiene Sesquiterpene γ-curcumene Sesquiterpene ar-curcumene 1.86 [3.91] Sesquiterpene α-zingiberene Sesquiterpene Unknown Sesquiterpene β-bisabolene Sesquiterpene β-curcumene 0.82* [0.75]* Sesquiterpene Unknown [0.82]* Oxygenated sesquiterpene Unknown 0.01 Oxygenated sesquiterpene β-sesquiphellandrene * Sesquiterpene Selina-4(15),7(11)-diene Sesquiterpene (E)-γ-Bisabolene 0.11 [0.75]* Sesquiterpene Selina-3,7(11)-diene 0.06 [3.91]* Sesquiterpene Unknown 0.20* 0.03 Sesquiterpene cis-sesquisabinene hydrate [0.20]* 0.15 Sesquiterpenic alcohol Unknown Oxygenated sesquiterpene (E)-Nerolidol Sesquiterpenic alcohol Unknown 0.13 Unknown Unknown 0.27* 0.19 Oxygenated sesquiterpene Caryophyllene oxide [0.27]* 0.05 Sesquiterpenic ether Caryophyllene oxide isomer [0.27]* tr Sesquiterpenic ether ar-turmerol 0.83 [0.85]* Sesquiterpenic alcohol trans-sesquisabinene hydrate 0.30* 0.20 Sesquiterpenic alcohol Unknown [0.30]* Oxygenated sesquiterpene ar-dihydroturmerone Sesquiterpenic ketone cis-zingiberenol Sesquiterpenic alcohol trans-zingiberenol 0.58 [1.28]* Sesquiterpenic alcohol Unknown Oxygenated sesquiterpene Unknown * Oxygenated sesquiterpene ar-turmerone 45.29* Sesquiterpenic ketone α-turmerone [45.29]* Sesquiterpenic ketone Unknown [45.29] [0.45]* Oxygenated sesquiterpene Unknown Oxygenated sesquiterpene β-turmerone * Sesquiterpenic ketone (Z)-α-Atlantone 0.22 [16.02]* Sesquiterpenic ketone Curcuphenol Sesquiterpenic alcohol (6S,7R)-Bisabolone Sesquiterpenic ketone Xanthorizzhol? 1.28 Sesquiterpenic alcohol Unknown 1.05 [0.91]* Oxygenated sesquiterpene Unknown 1.14 Oxygenated sesquiterpene (E)-α-Atlantone Sesquiterpenic ketone Page 4/13

6 Unknown Oxygenated sesquiterpene Unknown Oxygenated sesquiterpene Unknown 1.55 Unknown Unknown Oxygenated sesquiterpene Unknown 0.12 Oxygenated sesquiterpene Unknown 0.13 Oxygenated sesquiterpene Unknown 0.10 Oxygenated sesquiterpene Unknown 0.63 Oxygenated sesquiterpene Unknown 0.05 Oxygenated sesquiterpene Unknown 0.04 Oxygenated sesquiterpene Unknown 0.45 Oxygenated sesquiterpene Unknown 0.66 Oxygenated sesquiterpene Unknown Oxygenated sesquiterpene Unknown 0.05 Oxygenated sesquiterpene Total identified 75.87% 79.68% *: Two or more compounds are coeluting on this column [xx]: Duplicate percentage due to coelutions, not taken account in the identified total tr: The compound has been detected below 0.005% of total signal. Note: no correction factor was applied Page 5/13

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10 FULL ANALYSIS DATA Identification Column DB-5 Column DB-WAX R.T R.I % R.T R.I % Toluene * α-thujene tr 1.54* 1003 [0.01] α-pinene Camphene 3.20* α-fenchene 3.20* 940 [0.01] tr Sabinene tr tr 6-Methyl-5-hepten-2- one tr tr Myrcene α-phellandrene Δ3-Carene α-terpinene para-cymene Limonene 4.49* ,8-Cineole 4.49* 1024 [0.85] (Z)-β-Ocimene tr tr (E)-β-Ocimene tr γ-terpinene Terpinolene Linalool Unknown [m/z 95, 150 (45), 110 (35), tr 107 (23), 109 (21)] 55 (30), 135 (15), tr tr (12), 150 (12)] cis-para-menth-2-en- 1-ol Camphor Unknown [m/z 95, 43 (74), 109 (72), 82 (62), (50) (14)] Borneol * δ-terpineol * Terpinen-4-ol Methylacetophenone para-cymen-8-ol α-terpineol * 1652 [0.06] α-phellandrene epoxide trans-piperitol * Unknown [m/z 43, 111 (88), 126 (74), (61) ? (2)] Unknown [m/z 137, 152 (28), 43 (25), 91 (24), 109 (23), tr Page 9/13

11 (19)] Carvotanacetone tr tr Unknown [m/z 93, 43 (60), 108 (58), 69 (36), 41 (35) (5), (1)] Thymol * Carvacrol * Unknown [m/z 111, 126 (93), 43 (90), 71 (60)...] Unknown [m/z 43, 97 (99), 107 (47), 41 (35), 55 (30)...] Unknown [m/z 133, 105 (45), 91 (38), 119 (36) (3)] Unknown [m/z 105, 91 (66), 120 (53), 93 (48), 77 (47), 71 (46), 43 (37), 145 (22) (2)] Unknown [m/z 119, 161 (36), 43 (33), 176 (26), 91 (24), 105 (22)] Unknown [m/z 43, 105 (50), 120 (40), 145 (36), 119 (33), 93 (30) (6)] Unknown [m/z 71, 100 (92), 111 (79), 69 (46), 109 (45)...] Unknown [m/z 119, 93 (83), 91 (51), 77 (32), 41 (31) (16)] tr Acora-3,7(14)-diene (Z?)-Vestitenone, or analog tr β-caryophyllene * α-santalene (trans?)-6- Hydroxypiperitone (cis?)-6- Hydroxypiperitone * γ-elemene trans-α-bergamotene * 1548 [0.41] Unknown [m/z 91, 129 (98), 143 (94), 185 (81), 200 (76)...] (E)-Vestitenone α-humulene Page 10/13

12 Unknown [m/z 69, 91 (78), 109 (54), 202 (43), 41 (36), 120 (34)] * 1626 [0.02] (E)-β-Farnesene β-acoradiene γ-curcumene ar-curcumene [3.91] α-zingiberene Unknown [m/z 119, 91 (14), 120 (10), 117 (9) ? (2)] β-bisabolene β-curcumene 11.62* * 1700 [0.75] Unknown [m/z 93, 91 (62), 119 (57), 105 (44), 43 (43) ? (1)] Unknown [m/z 121, 93 (56), 91 (12), 94 (11), 122 (10)...220] 11.62* 1508 [0.82] β- Sesquiphellandrene * Selina-4(15),7(11)- diene (E)-γ-Bisabolene * 1700 [0.75] Selina-3,7(11)-diene * 1729 [3.91] Unknown [m/z 91, 93 (82), 79 (79), 77 (68), 67 (55), 41 (49) (12)] cis-sesquisabinene hydrate Unknown [m/z 138, 96 (100), 95 (85), 109 (74), 110 (60), 105 (57) (10)] 12.10* * 1546 [0.20] (E)-Nerolidol Unknown [m/z 109, 67 (30), 43 (24), 91 (19)...] Unknown [m/z 105, 83 (65), 120 (41), 119 (35), 91 (29), 55 (22) ? (t)] 12.43* Caryophyllene oxide 12.43* 1572 [0.27] Caryophyllene oxide isomer 12.43* 1572 [0.27] tr ar-turmerol * 2159 [0.85] trans-sesquisabinene hydrate 12.61* Unknown [m/z 43, 91 (63), 41 (61), 79 (58), 12.61* 1586 [0.30] Page 11/13

13 95 (49), 93 (47) (36)...] ar-dihydroturmerone cis-zingiberenol trans-zingiberenol * 2133 [1.28] Unknown [m/z 120, 91 (29), 43 (25), (23), 77 (21) (1)] 109 (45), 43 (43), 55 (36), 41 (35) (3)] * ar-turmerone 13.74* α-turmerone 13.74* 1678 [45.29] Unknown [m/z 69, 41 (90), 123 (74), 122 (51) (14), 218 (2)] Unknown [m/z 199, 132 (99), 59 (25), 91 (20), 117 (19), 43 (14) ? (6)] [45.29] 16.00* 2203 [0.45] β-turmerone * (Z)-α-Atlantone * 2184 [16.02] Curcuphenol (6S,7R)-Bisabolone Xanthorizzhol? (21), 43 (13), 41 (9), 91 (8) ? (1)] Unknown [m/z 119, 83 (30), 91 (14), 120 (11), 114 (9) (1)] * 2382 [0.91] (E)-α-Atlantone Unknown [m/z 95, 136 (77), 83 (68), 125 (55), 109 (40), 110 (39) (7)] Unknown [m/z 69, 43 (95), 41 (84), 109 (78), 95 (54), 93 (49) (36), 220 (2)...] 121 (74), 114 (74), 93 (40), 55 (20), 91 (20)...] 119 (56), 95 (53), 234 (32), 91 (30)] 136 (51), 43 (46), 109 (29), 55 (26), 119 (22) (1)] Page 12/13

14 136 (36), 55 (26), 109 (21), 79 (17), 43 (16) (11)] 107 (37), 43 (32), (30), 55 (27)...] 43 (24), 55 (21), 109 (15), (14) (1)] 55 (15), 43 (14), 91 (8) (1)] 149 (24), 135 (24), (24), 43 (13) (3)] 55 (18), 43 (14), 84 (6) (t)] 55 (22), 43 (22), 119 (19), (10)...] 43 (22), 55 (20), 98 (14), (14)...] 135 (32), 55 (26), 125 (18), 41 (13), (12) (1)] Total identified 75.87% 79.68% Total reported 89.50% 84.09% *: Two or more compounds are coeluting on this column [xx]: Duplicate percentage due to coelutions, not taken account in the identified total : Peaks apexes were resolved, but peaks overlapped and were summed for analysis tr: The compound has been detected below 0.005% of total signal. Note: no correction factor was applied R.T.: Retention time (minutes) R.I.: Retention index Page 13/13