Mosaic. Mosaic Real Time Parallel Illumination of Multiple Regions. Features and Benefits. DMD Technology. Specifications Summary

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1 Active Illumination Features and Benefits Unlimited flexibility in shape, size and complexity of illumination mask Simultaneous illumination of multiple regions of interest Precise illumination of areas of interest that protects target specimen and fluorophore Zero delta acquisition time for true digital excitation/illumination Longest lifetime and lowest maintenance with rugged semiconductor device DMD Technology The core of Mosaic is the Digital Micromirror Device (DMD), a high speed and highly efficient semiconductor-based light switch array of hundreds of thousands hinge-mounted, addressable, tiltable, microscopic mirrors. When a DMD chip is coordinated with a digital video or graphic signal, a light source, and beam delivery optics, its mirrors reflect a digital image of the illumination mask onto the sample. Mosaic Real Time Parallel Illumination of Multiple Regions Mosaic is a patented instrument platform built around MEMS Digital Mirror Devices (DMD). Mosaic exploits DMD in a proprietary programmable platform, integrated with scientific light sources including lasers, LEDs and arc lamps, and operates from 360 to 800 nm. It is offered with a range of high performance microscope adapter optics and can be integrated with CLSM, spinning disk and wide field imaging modalities. High speed frame switching (60 Hz) makes Mosaic suitable for many dynamic applications including bleaching, uncaging, photo-switching, optogenetics and constrained illumination. Variable intensity distributions can be achieved by rapid gating of mirror patterns. Specifications Summary Transmission Extinction ratio > 1000:1 Minimum resolvable spot Optical pixel rise / fall time < 1 µs Minimum optical pulse width Maximum frame repetition rate 360 nm to 800 nm Diffraction limited with 60x or 100x objective 75 µs external trigger 100 µs internal trigger 600 frames / sec Certification CDRH IIIb (if fitted with a laser source) Page 1 of 9

2 Hardware Specifications Optical Model Mosaic Mosaic Transmission Intensity stability 360 nm to 800 nm Absolute Extinction ratio > 1000:1 Minimum resolvable spot Fields of view Illumination port clear aperture Diffraction limited with 100x objective 7206 = 5.4 x 4.1 mm 7201 = 15.2 x 11.3 mm Ø 34 mm For flat field illumination port options, please see table at bottom of this section Illumination Source Options DMD illumination ports 1 or 2 on left hand side* 1 or 2 on left hand side, 1 or 2 on right hand side* Incoherent sources High pressure mercury lamp (100 W - 300W) DG4 Andor AMH-200 Other light sources on request CW laser 405 nm diode 488 nm Argon 514 nm Argon Other on request * 7201 allows only 1 port, 7206 allows 2 ports Additional illumination source options available on request Illumination Port Options Dichroic beamsplitter Single pass, specify wavelength Multi-pass, specify wavelength Beamsplitter 360 nm to 700 nm, R = 100, 70, 50 or 30% Excitation filter * Laser safety filter, interlocks 480 nm / 20 nm (GFP), 470 nm / 40 nm (FITC) - 38 mm Other filters on request - 25 mm with adapter Laser specific Optional * Additional excitation filter options available on request Flat field EPI illumination ports Adapter Leica DMI series Nikon TE2000 Nikon Ti series Nikon Universal Olympus IX series Part Number MM-7310-DMI-FFEP-X* MM-7310-TE2K-FFEP-X* MM-7310-TI-FFEP-X* MM-7310-NFS-FFEP-X* MM-7310-IX70-FFEP-X* Adapter Zeiss Axiovert 200 Zeiss Axio Examiner D1 Zeiss Axio Imager M1 Zeiss Axio Observer C-mount video port Part Number MM-7310-ZSAV-FFEP-X* MM-7310-D1-FFEP-X* MM-7310-IMG-FFEP-X* MM-7310-OBS-FFEP-X* On request *For 7201, X = 1 *For 7206, X = 2 INTEROPERABILITY Compatible with microscopes platforms - point & slit scan confocal, spinning disk confocal & widefield. Custom OEM systems available for High Content Screening and other fluorescence based imaging instrumentation. Compatible with control and acquisition software from market leading microscope manufacturers including Carl Zeiss, Leica and Olympus. Wavelength modularity - add lasers and filters cost effectively. Page 2 of 9

3 Software Specifications The Mosaic system includes software that controls calibration and enables the user to graphically or numerically define and edit the shape and characteristics of the illumination at the target. Release MicroPoint Mosaic Version 1.1 Drivers Calibration Operating systems File Functions Drawing Editor Shapes Drawn Shape Characteristics Controls Options PLX 9054 API version 4.1 for Mosaic.exe UI PLX 9054 API version 6 for SDK Scale & Offset corrections for X & Y 8 user-defined descriptions Windows 2000, XP Import (*.dxf, *.bmp, *.msk); Save *.msk Line, Paintbrush, Rectangle, Oval, Freehand polyshape Line width, paintbrush size, Alpha blended color and opacity of defined regions, Color palette Click and drag shapes and edges, cut, copy, paste, delete shape, Group, ungroup shapes Engage mask pattern, Go dark (global), Stop, Snapshot video Continuous update from drawing editor, Configure video subsystem, Exposure time & trigger selection Video mode & time selection, On/Off, Adjust laser outline highlight The angular alignment of either the CW laser or lamp illumination is factory set. The spatial alignment is controlled via the software calibration routine. The focus in the z direction is fixed at installation or OEM integration. Beam shaping is then controlled with the spatial light modulating DMD. Define region of interest and excitation energy at the target in image acquisition software Simultaneously excite and acquire image with zero delta time Software Compatibility - Life Science Applications Mosaic systems are compatible with a wide range of life science imaging software as indicated in the table below. Andor iq Version 2.0 MicroPoint Laser Galvo Version 1.0 MDC MetaMorph Ver 7.1 and above (32-bit) MDC MetaMorph Ver 7.74 and above (64-bit) Nikon NIS Elements 3.0 SP4 Build 505 Software Development Kits (SDK) Live cell multi-dimensional imaging with flexible control of MicroPoint Image-based targeting, control & calibration Fully integrated MicroPoint support with powerful imaging capabilities Fully integrated MicroPoint support with powerful imaging capabilities Multi-dimensional imaging with simple/fast control of MicroPoint SDKs are available for OEM to develop software to control integrated MicroPoint systems Page 3 of 9

4 Components The main components of Mosaic are shown below: Beamsplitter/ dichroic plugs Flange for EPI source DMD array Electronic interface Laser fiber input Laser zoom collimator Flange for optional second light source Page 4 of 9

5 Ordering your Mosaic system Prior to commencing the order process, please advise your customer representative of your application requirements. Please also refer to the flow charts on the next 2 pages. Step 1. Confirm your Microscope make and model Leica, Nikon, Olympus, Zeiss Step 2. The following field of illumination options are available: 7201 = 15.2 x 11.3 mm and 7206 = 5.4 x 4.1 mm Step 1. In order to allow us to select the correct model for your requirements please advise your customer representative of the make, model (and any accessories fitted) of the microscope being used. Step 2. Please select the field of illumination required. Step 3a. Select the primary left sided DMD light source. For the 7206 option only, a secondary left side light source can also be selected at this point. Step 3b. For Mosaic systems only a primary right sided light source can also be selected. For the 7206 option only, a secondary right side light source can also be selected at this point. Step 4. Select the type of EPI light source require. Step 5. Step 3a. The following DMD light sources / filter sets for the left side port of Mosaic are available: Diode laser, XLED1, Other laser, Andor AMH, DG4, Hg The following left sided excitation filters are available: GFP filter in holder (480/20), FITC (470/40),Blank filter holder, Others on request Secondary left sided DMD source, select one or more of the following beamsplitters: 30 EPI, 50 EPI,70 EPI, 100% EPI Plug, 0% EPI (Blank) Plug. A range of dichroic mirrors is available on request. Step 3b. For Mosaic systems only, additional DMD light sources / filter sets for the right side port: Diode laser, XLED1, Other laser, Andor AMH, DG4, Hg The following right sided excitation filters are available: GFP filter in holder (480/20), FITC (470/40),Blank filter holder, Others on request Secondary right sided DMD source, select one or more of the following beamsplitters: 30 EPI, 50 EPI, 70 EPI, 100% EPI Plug, 0% EPI (Blank) Plug. A range of dichroic mirrors is available on request. Step 4. The following EPI light sources are available: Andor AMH, XLED1, DG4, Microscope specific Hg lamp, MicroPoint, other Step 5. Optional EPI Excitation filters are also available: GFP filter in holder (480/20), FITC (470/40). Others on request Step 6. The following EPI-Mosaic beamsplitters are available: 30 EPI, 50 EPI, 70 EPI, 100% EPI plug, 0% EPI (Blank) Plug Choose one or more optional EPI excitation filters. Step 6. Choose one or more EPI / Mosaic beamsplitters. Page 5 of 9

6 Configuring Mosaic The Mosaic can be tailored to suit your needs, there are a number of options including mosaic illumination sources, EPI illumination sources, Active mirror array magnification and filtering. Microscope Olympus Nikon Zeiss Leica Microscope Options Mosaic Illumination Input with & BNC Cable Laser Delivery / safety filters Three Channel TTL synchronization modules with delay and duration inputs/outputs Field of Illumination x 11.3mm 5.4 x 4.1mm Mosaic Input Light Source Diode laser XLED1 AMH DG4 Hg Other Note: If laser chosen, then laser kit (containing fluorescent target, fiber coupler, homogenizer, fiber collimator & interlock) is required Input Excitation Filter Large GRP filter in holder (480 x 40nm) Large blank filter holder Secondary Light Source Beamsplitters (Choose one or more) 30 EPI:70 Laser Beamsplitter Plug 50 EPI:50 Laser Beamsplitter Plug 70 EPI:30 Laser Beamsplitter plug 100% EPI Plug 0% EPI (Blank) Plug EPI Light Source AMH DG4 XLED1 Hg MicroPoint Other 5 Optional EPI Excitation Filters (Choose one or more if required) FITC exciter filter GFP exciter filter Rhodamine exciter filter Blank filter holder EPI/ Mosaic Beamsplitter 30 EPI:70 Laser Beamsplitter Plug 100% EPI Plug 50 EPI:50 Laser Beamsplitter Plug 0% EPI (Blank) Plug 70 EPI:30 Laser Beamsplitter Plug Multiplex with MicroPoint For applications requiring both ablation and zero delta time fluorescence excitation of multiple regions with complex geometries, the Mosaic s EPI Illumination port can be fitted with a Micropoint system and filtering. Page 6 of 9

7 Configuring Mosaic (Complementary Multicolour Illumination) Some photosensitive proteins can be activated with one wavelength of light and deactivated or silenced with another. This property is exploited in the Mosaic e.g. to sharpen control over protein localization. A second light source is coupled into the DMD at a complementary angle to the primary. In this configuration pixels NOT selected for activation define an inverse mask which can be illuminated to silence molecules outside of the activation region(s) or those diffusing away. Microscope Olympus Nikon Zeiss Leica Microscope Options Mosaic Illumination Input with & BNC Cable Laser Delivery / safety filters Three Channel TTL synchronization modules with delay and duration inputs/outputs Field of Illumination x 4.1mm Primary Left Sided Light Source Diode laser XLED1 AMH DG4 Hg Other Note: If laser chosen, then laser kit (containing fluorescent target, fiber coupler, homogenizer, fiber collimator & interlock) is required Left Side Excitation Filter Large GRP filter in holder (480 x 40nm) Large blank filter holder Secondary Left Side Light Source Beamsplitters (Choose one or more) 30 EPI:70 Laser Beamsplitter Plug 50 EPI:50 Laser Beamsplitter Plug 70 EPI:30 Laser Beamsplitter plug 100% EPI Plug 0% EPI (Blank) Plug Primary Right Sided Light Source Diode laser XLED1 AMH DG4 Hg Other Note: If laser chosen, then laser kit (containing fluorescent target, fiber coupler, homogenizer, fiber collimator & interlock) is required Right Side Excitation Filter Large GRP filter in holder (480 x 40nm) Large blank filter holder EPI Light Source AMH DG4 XLED1 Hg MicroPoint Other Secondary Right Side Light Source Beamsplitters (Choose one or more) 30 EPI:70 Laser Beamsplitter Plug 50 EPI:50 Laser Beamsplitter Plug 70 EPI:30 Laser Beamsplitter plug 100% EPI Plug 0% EPI (Blank) Plug EPI/ Mosaic Beamsplitter 30 EPI:70 Laser Beamsplitter Plug 100% EPI Plug 50 EPI:50 Laser Beamsplitter Plug 0% EPI (Blank) Plug 70 EPI:30 Laser Beamsplitter Plug 5 Optional EPI Excitation Filters (Choose one or more if required) FITC exciter filter GFP exciter filter Rhodamine exciter filter Blank filter holder Page 7 of 9

8 Product Drawings Dimensions in mm [inches] Fields of Illumination (FoI) [3.8] 80 [3.1] Model FOV (Lamp house) Diagonal, mm [3.0] 0 FOV (Lamp house) XY, mm 15.2 x x 4.1 FOV 0.4 mm fiber ZC* x2 (mm)** 13.7 x x [3.8] FOV 0.4 mm fiber ZC x1 (mm)*** FOV 0.2 mm fiber ZC x2 (mm)** FOV 0.2 mm fiber ZC x1 (mm)** [5.8] [4] Fiber Bend Radius Laser Fiber Collimator Mosaic Lamphouse Adapter 175 [6.88] (Lamphouse Attachment Plane) DMD effective pixel size (µm) *ZC = Zoom Collimator X2 = max; X1 = min ** Intersection of circle and DMD rectangle *** Circular field of illumination Moves 38.1[1.50] Removeable Beamsplitter 57 [2.2] Ø Cylinder Moves 38.1[1.50] 76.2 [3.0] 0 See Table Weights: Mosaic Body = 3.5 kg [7.5 lb] 160 [6.3] Mosaic Body = 4.5 kg [9.8 lb] [3.1] Body Dimensions Model Part Number Length 7201 MEC mm [14.53] 76.2 [3.0] MEC mm [13.1] (Laser Input Attachment) 146 [5.8] [4] Fiber Bend Radius Mosaic Lamphouse Adapter 160 [6.3] 229 [9.0] 240 [9.375] (Lamphouse Attachment Plane) Laser Fiber Collimator Removeable Beamsplitter 57 [2.2] Ø Cylinder Moves 38.1[1.50] Moves 38.1[1.50] 76.2 [3.0] [13.1] Page 8 of 9

9 Order Today Need more information? At Andor we are committed to finding the correct solution for you. With a dedicated team of technical advisors, we are able to offer you one-to-one guidance and technical support on all Andor products. For a full listing of our regional sales offices, please see: /contact Our regional headquarters are: Europe Belfast, Northern Ireland Japan Tokyo Phone +44 (28) Phone +81 (3) Fax +44 (28) Fax +81 (3) North America China Connecticut, USA Beijing Phone +1 (860) Phone +86 (10) Fax +1 (860) Fax +86 (10) Items shipped with your Mosaic: 1x DMD Head assembly, Mosaic II 1x PLX and daughter board pair, programmed 1x 10 SCSI-3 Ultra LVD/SE MD698 cable 1x TPES15-T050PP-7 Mosaic custom power supply 1x 3m mains cable for TPES15-T050PP-7 1x First surface mirror 10x Green Post-It notes 1x Allen key (0.05 ) 1x T-handle (3/32) 1x LED Maglite, with 2 xaa batteries 1x Maglite clamp 1x Mosaic post holder 1x Mosaic foot 1x NT stainless steel post (1/4-20 UNC) 1x NT stainless steel post (1/4-20 UNC) 1x Zeiss male flange 1x BNC finger assembly 1x User guide NOTE: Laser radiation is only present in Mosaic systems fitted with a laser. Some Mosaic systems use non laser-based light sources. Minimum Computer Requirements: The imaging computer must have two adjacent PCI slots (with exceptions) and a bay for BNC Trigger connectors. Application software for the Mosaic Digital Diaphragm System is typically integrated into the imaging software provided for the microscope. Operating & Storage Conditions Operating Temperature: 0 C to 30 C ambient Relative Humidity: < 70% (non-condensing) Storage Temperature -25 C to 50 C Power Requirements VAC, Hz, 1.0 A Windows is a registered trademark of Microsoft Corporation. MMosaicSS 0213 R1 Page 9 of 9